Perforated dried blood spot accurate microsampling: the concept and its applications in toxicokinetic sample collection

Journal of Mass Spectrometry : JMS
Fumin LiSteve Michael

Abstract

Dried blood spot (DBS) sampling has gained considerable interest as a microsampling technique to support drug discovery and development owing to its enormous ethical and practical benefits. Quantitative determinations of drugs and/or their metabolites collected in DBS matrix in its current format, however, have encountered technical challenges and regulatory uncertainty. The challenges of DBS bioanalysis are largely ascribed to the way how samples are collected and analyzed. Currently, an uncontrolled amount of a blood sample, e.g. 20 µl, is collected per time point per sample and spotted onto cellulose paper. Quantitation is based on removal of a fixed area of the DBS sample, resulting in sample waste, a need for mechanical punching and concomitant potential punching carryover, uncertainty in recovery assessment and the adverse impact of hematocrit on accurate quantitation. Here, we describe the concept and applications of a novel concept, namely perforated dried blood spot (PDBS), for accurate microsampling that addresses previous challenges. Advantages of PDBS are enumerated and compared with conventional DBS in the context of microsampling and liquid chromatography tandem mass spectrometry bioanalysis. Two approaches for ac...Continue Reading

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Oct 21, 2011·Bioanalysis·Fumin LiSteve Michael

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Citations

Aug 17, 2011·World Journal of Emergency Surgery : WJES·Joseph S HannaReza Askari
Aug 19, 2014·Analytical and Bioanalytical Chemistry·Markus R Meyer
Aug 28, 2014·Bioanalysis·Pieter M M De KeselChristophe P Stove
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Nov 23, 2012·Analytical Chemistry·Plamen A Demirev
Oct 17, 2021·Scientific Reports·Kelly WhittakerRuo-Pan Huang

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