PMID: 7517634Jun 1, 1994Paper

Perforated-patch recording does not enhance effect of 3-isobutyl-1-methylxanthine on cardiac calcium current

The American Journal of Physiology
A Kawamura, Gordon M Wahler

Abstract

Conventional whole cell voltage-clamp recording results in washout of the cardiac Ca2+ current (ICa) response to the beta-adrenergic agonist isoproterenol (Iso), for reasons which are not clear. When dose-response curves for the phosphodiesterase (PDE) inhibitor 3-isobutyl-1-methylxanthine (IBMX) were compared using perforated-patch vs. conventional whole cell recording in guinea pig ventricular myocytes, the conventional whole cell IBMX responses were unexpectedly larger than the perforated-patch responses. Furthermore, during conventional whole cell recording the response to repeated application of Iso declined rapidly, whereas the IBMX response initially increased and then declined. When pipette [Ca2+] was increased to 10(-7) M, conventional whole cell responses to 300 microM IBMX and 10(-9) M Iso were identical to perforated-patch responses. Thus loss of the Iso response during conventional whole cell recording seems to not be solely due to a washout of some constituent of the adenosine 3',5'-cyclic monophosphate pathway. We suggest that unphysiological intracellular [Ca2+] enhances the relative PDE activity and that this contributes to the rapid decline of the Iso response and the initial enhancement of the IBMX response.

References

Jan 1, 1991·Life Sciences·R Fischmeister, H C Hartzell
Nov 1, 1991·Pflügers Archiv : European journal of physiology·A CavaliéW Trautwein
Apr 1, 1991·Circulation Research·D C BodeL L Brunton
Mar 1, 1991·Journal of Neuroscience Methods·J RaeM Watsky
Jan 1, 1990·The Japanese Journal of Physiology·K YazawaM Kameyama
Oct 1, 1985·Pflügers Archiv : European journal of physiology·M KameyamaW Trautwein
Aug 1, 1986·Pflügers Archiv : European journal of physiology·J HeschelerW Trautwein
Aug 1, 1988·The Journal of General Physiology·R Horn, A Marty
Apr 1, 1989·Pflügers Archiv : European journal of physiology·R C LeviR Fischmeister
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Feb 1, 1983·The Journal of General Physiology·J R Hume, W Giles
Feb 1, 1984·Circulation Research·I R JosephsonA M Brown
Sep 1, 1993·Pflügers Archiv : European journal of physiology·I Chung, L C Schlichter
Feb 1, 1993·Trends in Pharmacological Sciences·D M Cooper, G Brooker

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Citations

Dec 19, 2001·American Journal of Physiology. Heart and Circulatory Physiology·Vida M VizgirdaDorie W Schwertz
Feb 14, 2006·Experimental Physiology·Dan MengJi-Nan Zhang
Mar 6, 2003·The Japanese Journal of Physiology·Michiko TashiroMasato Konishi
Aug 13, 1999·Journal of Cardiovascular Pharmacology·R SakaiA J Pappano
Jan 17, 2007·Molecular and Cellular Biochemistry·Jacquelyn M SmithGordon M Wahler
Jul 3, 2002·Biochemical Pharmacology·Timothy P GeisbuhlerHeather D Ervin

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