Perforin affects regeneration in a mouse spinal cord injury model.

The International Journal of Neuroscience
Igor Jakovcevski, Melitta Schachner

Abstract

Locomotor outcomes in perforin-deficient (Pfp-/-) mice and wild-type littermate controls were measured after severe compression injury of the lower thoracic spinal cord up to six weeks after injury. According to both the Basso mouse scale score and single frame motion analysis, motor recovery of Pfp-/- mice was similar to wild-type controls. Interestingly, immunohistochemical analysis of cell types at six weeks after injury showed enhanced cholinergic reinnervation of spinal motor neurons caudal to the lesion site and neurofilament-positive structures at the injury site in Pfp-/- mice, whereas numbers of microglia/macrophages and astrocytes were decreased compared with controls. We conclude that, although, loss of perforin does not change the locomotor outcome after injury, it beneficially affects diverse cellular features, such as number of axons, cholinergic synapses, astrocytes and microglia/macrophages at or caudal to the lesion site. Perforin's ability to contribute to Rag2's influence on locomotion was observed in mice doubly deficient in perforin and Rag2 which recovered better than Rag2-/- or Pfp-/- mice, suggesting that natural killer cells can cooperate with T- and B-cells in spinal cord injury.

References

Jan 1, 1990·Annual Review of Immunology·J Tschopp, M Nabholz
Jan 1, 1985·Annual Review of Immunology·P A Henkart
Aug 1, 1995·Immunological Reviews·C C LiuJ D Young
Sep 21, 2000·The Journal of Clinical Investigation·A ShustovC S Via
Jun 18, 2004·Progress in Neurobiology·Karim Fouad, Keir Pearson
Nov 13, 2004·Cerebral Cortex·Andrey IrintchevMelitta Schachner
Nov 30, 2004·Trends in Molecular Medicine·Michael S Beattie
Oct 26, 2005·Neuromolecular Medicine·Ingo Bechmann
Apr 25, 2006·Journal of Neurotrauma·Michal Schwartz, Eti Yoles
Jul 22, 2006·Nature Reviews. Neuroscience·Sandrine ThuretFred H Gage
Jul 28, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ivayla ApostolovaMelitta Schachner
Nov 16, 2006·Glia·Alain BessisAnne Roumier
Dec 5, 2006·Trends in Immunology·Ingo BechmannV Hugh Perry
Feb 9, 2007·Proceedings of the National Academy of Sciences of the United States of America·Gareth B MilesRobert M Brownstone
Mar 30, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Masayuki HashimotoWise Young
Jul 6, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Igor JakovcevskiMelitta Schachner
Dec 7, 2007·Clinical Neuroscience Research·Alpa TrivediLinda J Noble-Haeusslein
Aug 30, 2008·PloS One·Georgette L SuidanAaron J Johnson
Nov 11, 2008·Recent Patents on CNS Drug Discovery·Carmen C M Chan
May 22, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Annika LieberothRalf Kleene
Jul 9, 2009·Nature Medicine·Phillip Popovich, Dana McTigue
Aug 15, 2009·Journal of Neuropathology and Experimental Neurology·Chandra DebCharles L Howe
Oct 15, 2009·Cardiology in the Young·Guo Chun-yanHan Xiu-zhen
Oct 15, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Ali MehannaMelitta Schachner
Jun 16, 2011·Nature Reviews. Neuroscience·Samuel David, Antje Kroner
Jan 22, 2013·Experimental Neurology·A M Laliberte, M G Fehlings
May 4, 2013·Neurobiology of Disease·Nevena DjogoMelitta Schachner
May 25, 2013·Journal of Anatomy·Emily C WittsGareth B Miles
Sep 24, 2013·Journal of Neuroinflammation·Fabiola PuentesSandra Amor
Jun 27, 2014·Experimental Neurology·Ali MehannaIgor Jakovcevski
Dec 17, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Eva MracskoRoland Veltkamp
May 23, 2015·Nature Reviews. Immunology·Ilia VoskoboinikJoseph A Trapani
May 15, 2016·The European Journal of Neuroscience·Vedangana SainiIgor Jakovcevski
Sep 1, 2016·Journal of Neuroinflammation·Robin C WillenbringAaron J Johnson
Feb 1, 2017·Biochemical and Biophysical Research Communications·Su-Ping PengYan-Qin Shen

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Citations

Feb 16, 2021·Neuroscience·Igor JakovčevskiMelitta Schachner
Mar 23, 2021·Experimental Neurology·Jonathon Chon Teng ChioMichael G Fehlings

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