Perforin hyperreleasability and depletion in cytotoxic T cells from patients with exacerbated atopic dermatitis and asymptomatic rhinoconjunctivitis allergica

The Journal of Allergy and Clinical Immunology
A AmbachH Gollnick

Abstract

As a plasma membrane pore-forming protein, perforin is essential for T-cell cytotoxicity mediated by lytic granules. Recent studies on the immune system of perforin knockout mice demonstrated striking similarities to the immunopathology of atopic diseases. We sought to investigate the perforin system of atopic patients. Monoclonal antibodies were used to characterize perforin-positive PBMCs of patients with exacerbated atopic dermatitis (AD) and asymptomatic rhinoconjunctivitis allergica (RCA) by means of immunoflow cytometry. In addition, a perforin release assay was developed to quantify the velocity of ionomycin and phorbol 12-myristate 13-acetate-induced secretion of lytic granules. In atopic patients significantly fewer lymphocytes contained perforin-positive lytic granules compared with those of healthy control subjects (patients with AD: 14% +/- 5%, n = 13, P <.0001; patients with RCA: 24% +/- 5%, n = 9, P <.01; healthy control subjects: 33% +/- 11%, n = 13). Of all CD8(hi+) cytotoxic T lymphocytes (CTLs), only 18% +/- 9% and 17% +/- 12% were perforin-positive in patients with AD and RCA, respectively, compared with 44% +/- 13% in control subjects (P <.0001). In addition, perforin-positive CD8(hi+) CTLs of atopic patient...Continue Reading

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Citations

Nov 16, 2005·Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·Andreas AmbachHarald Gollnick
Jun 12, 2004·The Journal of Investigative Dermatology·Andreas AmbachHarald Gollnick
Feb 10, 2006·Journal of Photochemistry and Photobiology. B, Biology·Andreas AmbachHarald Gollnick
Mar 25, 2004·Nature Reviews. Immunology·Thomas S Kupper, Robert C Fuhlbrigge
Sep 6, 2011·Allergologia et immunopathologia·E KucukosmanogluA Balat

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