Performance assessment of a nested-PCR assay (the RAPID BAP-MTB) and the BD ProbeTec ET system for detection of Mycobacterium tuberculosis in clinical specimens

Journal of Clinical Microbiology
Jann-Yuan WangKwen-Tay Luh

Abstract

The performance of a nested PCR-based assay (the RAPID BAP-MTB; AsiaGen, Taichung, Taiwan) and the BD ProbeTec ET (DTB) system (Becton Dickinson, Sparks, Md.) for detection of Mycobacterium tuberculosis was evaluated with 600 consecutive clinical samples. These samples, including 552 respiratory specimens and 48 nonrespiratory specimens, were collected from 333 patients treated at National Taiwan University Hospital from September to October 2003. The results of both assays were compared to the gold standard of combined culture results and clinical diagnosis. The overall sensitivity and specificity of the RAPID BAP-MTB assay for respiratory specimens were 66.7% and 97.2%, respectively, and for the DTB assay they were 56.7% and 95.3%, respectively. The positive and negative predictive values for the RAPID BAP-MTB were 74.1% and 96.0%, respectively, and for the DTB assay they were 59.6% and 94.7%, respectively. For smear-negative samples, the sensitivity of the RAPID BAP-MTB and DTB assays was 57.1% and 40.5%, respectively. The RAPID BAP-MTB assay produced 14 false-positive results in 14 samples, including one of the six samples yielding Mycobacterium abscessus, one of the six samples yielding Mycobacterium avium intracellulare, ...Continue Reading

References

Apr 1, 1975·Annals of Internal Medicine·J C Boyd, J J Marr
Apr 1, 1980·Annals of Internal Medicine·P R MurrayD J Krogstad
Nov 1, 1996·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·J R DalovisioE S Cooper
Feb 3, 2000·Journal of Clinical Microbiology·J S BergmannG L Woods
Dec 20, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Sonia H MontenegroRichard A Oberhelman
May 14, 2003·Diagnostic Microbiology and Infectious Disease·Tsi-Shu HuangYung-Ching Liu
Dec 10, 2003·Journal of Clinical Microbiology·Claudio Piersimoni, Claudio Scarparo

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Citations

May 8, 2009·International Journal of Colorectal Disease·Pei-Ying LinPan-Chyr Yang
Jul 24, 2010·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·W-L LiuP-R Hsueh
Aug 22, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Chih-Cheng LaiPo-Ren Hsueh
Jan 16, 2007·Medicine·Jann-Yuan WangKwen-Tay Luh
Jan 16, 2010·Medicine·Chin-Chung ShuUNKNOWN Taiwan Anti-Mycobacteria Investigation (TAMI) Group
Oct 27, 2012·Critical Care : the Official Journal of the Critical Care Forum·Yu-Tzu TsengJann-Yuan Wang
Jun 8, 2007·Emerging Infectious Diseases·Jann-Yuan WangKwen-Tay Luh
Jun 30, 2011·Lung India : Official Organ of Indian Chest Society·K Lily ThereseH N Madhavan
Apr 17, 2009·Journal of the Formosan Medical Association = Taiwan Yi Zhi·Hsiang-Chi KungShan-Chwen Chang
Feb 20, 2013·Orthopaedic Surgery·Chia-liang AngSittampalam Kesavan
Feb 7, 2015·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M-R LeeC-J Yu
May 15, 2015·Analytical and Bioanalytical Chemistry·Danishmalik Rafiq SayyedTaisun Kim
Jun 26, 2012·The Brazilian Journal of Infectious Diseases : an Official Publication of the Brazilian Society of Infectious Diseases·Hung-Chang HungYuan-Ti Lee
Feb 14, 2006·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Claudine BékondiYves Germani
May 27, 2009·Respiratory Medicine·Chun-Ta HuangUNKNOWN Tami Group
Jun 3, 2011·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C-C ShuUNKNOWN TAMI group
Mar 15, 2016·Journal of Clinical Gastroenterology·Hongying GanLiangru Zhu
Dec 1, 2014·JMM Case Reports·Lily Therese KulandaiMadhavan Hajib Narahari Rao
Feb 6, 2017·Microbiology Spectrum·Wendy Susan StevensKeertan Dheda

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