Performance assessment of digital PCR for the quantification of GM-maize and GM-soya events

Analytical and Bioanalytical Chemistry
Geoffrey CottenetPoh Fong Chuah

Abstract

Accurate quantitative methods are needed to determine the amount of transgenic material in ingredients and comply with labelling GMO thresholds. Quantitative real-time PCR methods are usually applied for GMO quantification, but since a few years, digital PCR (dPCR) has been described as a potential alternative by quantifying DNA molecules directly without any standard curves. In this study, the performance of dPCR to quantify 9 GM-soya events and 15 GM-maize events was assessed. Following GMO validation guidelines, the trueness and precision were determined on high, medium and low levels of transgenic content. Results showed biases below ± 25% and satisfactory precision data. Limits of quantification were determined for each GM-event and were between 12 and 31 target copies. The reliability of GMO quantification by dPCR was further confirmed by analysing several proficiency test samples. Overall, dPCR showed accurate and precise GMO quantification on all the tested GM-events, from high to low transgenic amount. With its ease-of-use, dPCR was found to be an appealing alternative technology for routine GMO testing laboratories. Graphical abstract.

References

Oct 10, 2009·Analytical and Bioanalytical Chemistry·Philippe CorbisierKerry R Emslie
Feb 13, 2013·Transgenic Research·Maher ChaouachiKhaled Saïd
Mar 5, 2013·Journal of AOAC International·Laura BonfiniAlexandre Patak
Dec 19, 2016·Biomolecular Detection and Quantification·Alexandra S WhaleSvilen Tzonev
Oct 27, 2017·Analytical and Bioanalytical Chemistry·David DobnikJana Žel
Mar 27, 2018·Analytical and Bioanalytical Chemistry·Tigst Demeke, David Dobnik
Apr 21, 2018·Sensors·Phenix-Lan QuanEric Brouzes

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