Performance assessment of the DR. MTBC Screen assay and the BD ProbeTec ET system for direct detection of Mycobacterium tuberculosis in respiratory specimens

Journal of Clinical Microbiology
Jann-Yuan WangKwen-Tay Luh

Abstract

The performance of the DR. MTBC PCR-based assay and the BD ProbeTec ET Mycobacterium tuberculosis Complex Direct Detection (DTB) assay for the direct detection of Mycobacterium tuberculosis was evaluated using 1,066 consecutive clinical respiratory samples collected from 494 patients who did not have old cases of pulmonary tuberculosis and were not receiving antituberculosis treatment at National Taiwan University Hospital from January to February 2005. The results of both assays were compared to the "gold standard" of combined culture results and clinical diagnosis. The overall sensitivity and specificity of the DR. MTBC Screen assay were 56.6% and 98.9%, respectively, and of the DTB assay were 63.2% and 98.4%, respectively. The positive and negative predictive values for the DR. MTBC Screen assay were 84.5% and 95.4%, respectively, and for the DTB assay were 81.7% and 96.0%, respectively. The DR. MTBC Screen assay produced 11 false-positive results for 11 patients, including three samples yielding non-M. tuberculosis mycobacteria (one each for M. abscessus, a mixture of M. abscessus and M. chelonae, and unidentified non-tuberculosis mycobacteria). The DTB assay produced 15 false-positive results for 13 patients, including fiv...Continue Reading

References

Apr 1, 1975·Annals of Internal Medicine·J C Boyd, J J Marr
Apr 1, 1980·Annals of Internal Medicine·P R MurrayD J Krogstad
Oct 16, 1996·JAMA : the Journal of the American Medical Association·A Pablos-MéndezT R Frieden
Feb 3, 2000·Journal of Clinical Microbiology·J S BergmannG L Woods
May 14, 2003·Diagnostic Microbiology and Infectious Disease·Tsi-Shu HuangYung-Ching Liu
Dec 10, 2003·Journal of Clinical Microbiology·Claudio Piersimoni, Claudio Scarparo
Apr 21, 2005·Thrombosis and Haemostasis·Jann-Yuan WangKwen-Tay Luh

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Citations

Sep 16, 2010·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M InoueT Barkham
Feb 4, 2010·The American Journal of the Medical Sciences·Devrim DundarVolkan Dundar
Jun 26, 2012·The Brazilian Journal of Infectious Diseases : an Official Publication of the Brazilian Society of Infectious Diseases·Hung-Chang HungYuan-Ti Lee
Oct 21, 2011·Memórias do Instituto Oswaldo Cruz·Al-Habib Omar Said ToihirHerimanana Ramarokoto

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