Performance Characteristics of Different Anti-Double-Stranded DNA Antibody Assays in the Monitoring of Systemic Lupus Erythematosus

Journal of Immunology Research
M MahlerThierry Dervieux

Abstract

We sought to evaluate different anti-double-stranded DNA assays for their performance characteristics in monitoring disease activity fluctuations in systemic lupus erythematosus (SLE). 36 active SLE patients were followed monthly. At each study visit (total n = 371), blood was collected and disease activity was scored using the SELENA-SLEDAI (excluding anti-dsDNA or complement components) and by a physician's global assessment (PGA). Four anti-dsDNA tests were compared. Linear mixed-effects models with random intercept and fixed slopes were used to evaluate the relationship between the longitudinal fluctuations of disease activity and anti-dsDNA titers. At enrollment, positivity for QUANTA Lite and high-avidity anti-dsDNA assay was both 64% and significantly lower than anti-dsDNA positivity by QUANTA Flash (83%) and CLIFT (96%). Linear mixed-effects modeling indicated that the change in clinical SELENA-SLEDAI scores was associated with the titers of all anti-dsDNA with QUANTA Flash yielding the highest marginal R2 (0.15; p < 0.01). QUANTA Flash was the only anti-dsDNA assay significantly associated with the change in PGA (marginal R2 = 0.05; p < 0.01). These data indicate that anti-dsDNA antibodies determined by QUANTA Flash ha...Continue Reading

References

Sep 1, 1981·Journal of Clinical Pathology·S D SomerfieldR J Booth
Nov 1, 1982·Arthritis and Rheumatism·E M TanR J Winchester
Dec 16, 2005·The New England Journal of Medicine·Michelle PetriUNKNOWN OC-SELENA Trial
Feb 15, 2007·Nature Clinical Practice. Rheumatology·Michael Mahler, Marvin J Fritzler
Nov 21, 2007·Annals of the Rheumatic Diseases·Michelle Petri
Jul 8, 2008·Autoimmunity Reviews·Luis E MunozMartin Herrmann
Nov 4, 2015·Immunopharmacology and Immunotoxicology·Michael MahlerMarvin J Fritzler
Feb 28, 2016·Journal of Immunological Methods·Gabriella LakosThierry Dervieux

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