Performance comparison of capillary and agarose gel electrophoresis for the identification and characterization of monoclonal immunoglobulins

American Journal of Clinical Pathology
Christopher R McCuddenDavid G Grenache

Abstract

The objective of this study was to compare gel- and capillary-based serum protein electrophoresis methods to identify and characterize monoclonal immunoglobulins (M proteins). Five reviewers interpreted 149 consecutively ordered serum specimens following agarose gel electrophoresis (AGE), capillary electrophoresis (CE), immunofixation electrophoresis (IFE), and subtraction immunotyping (IT). As a screening test for detecting M proteins, AGE and CE displayed similar sensitivity (91% and 92%, respectively). CE was less specific (74%) than AGE (81%). An analysis of interinterpreter agreement revealed that interpretations were more consistent using gel-based methods than capillary-based methods, with 80% of the gel interpretations being in complete (5/5) agreement compared with 67% of the capillary interpretations. After implementing the capillary-based methods, the number of tests per reportable result increased (from 1.58 to 1.73). CE is an analytically suitable alternative to AGE, but laboratories implementing it will need to continue IFE testing to characterize all M proteins detected by CE.

Citations

Aug 26, 2014·Autoimmunity Reviews·Salim TradCamille Frances
Apr 3, 2012·Clinical Biochemistry·Jessica PoissonChristopher R McCudden
Jun 15, 2011·Clinical Biochemistry·Céline ChartierJean Paul Cristol
Nov 3, 2010·Veterinary Clinical Pathology·Roberto Vailati FacchiniPaola Roccabianca
Sep 30, 2016·Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis·Kanji Miyazaki, Kenshi Suzuki
Nov 14, 2016·Clinical Biochemistry·Cecilia SartoPaolo Brambilla
Dec 22, 2017·Archives of Pathology & Laboratory Medicine·Jonathan R GenzenMohammad Q Ansari
Mar 19, 2016·World Journal of Clinical Cases·Belkiz OngenIbrahim Unsal
Oct 15, 2013·Electrophoresis·Shuai Sherry Zhao, David D Y Chen
Dec 24, 2013·Annals of Clinical Biochemistry·Fehime Benli AksungarIbrahim Unsal

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