Performance comparison of EasyFix G26 and HYDRASYS 2 SCAN for the detection of serum monoclonal proteins.

Journal of Clinical Laboratory Analysis
Fatimah Diana Amin NordinYusnita Yakob

Abstract

Serum protein electrophoresis (SPE) is a widely used laboratory technique to diagnose patients with multiple myeloma (MM) and other disorders related to serum protein. In patients with MM, abnormal monoclonal protein can be detected by SPE and further characterized using immunofixation electrophoresis (IFE). There are several semi-automated agarose gel-based systems available commercially for SPE and IFE. In this study, we sought to evaluate the analytical performance of fully automated EasyFix G26 (EFG26) and semi-automated HYDRASYS 2 SCAN (H2SCAN) for both SPE and IFE. Both instruments were operated according to manufacturer's instructions. Samples used include a commercially available normal control serum (NCS) and patients' specimens. The following were evaluated: precision and comparison studies for SPE, and reproducibility and comparison studies for IFE. Statistical analyses were performed using Microsoft Excel. For SPE repeatability study, our results showed that EFG26 has higher coefficient of variation (%CV) compared with H2SCAN for both samples except for monoclonal component with %CV of 0.97% and 1.18%, respectively. Similar results were obtained for SPE reproducibility study except for alpha-1 (4.16%) and beta (3.13...Continue Reading

References

Dec 13, 2003·The Hematology Journal : the Official Journal of the European Haematology Association·Brian G M DurieUNKNOWN Scientific Advisors of the International Myeloma Foundation
Oct 7, 2006·Annals of Clinical Biochemistry·Marie HughesMark McColl
Feb 5, 2011·Blood·Meletios DimopoulosUNKNOWN International Myeloma Workshop Consensus Panel 3
Jun 15, 2011·Clinical Biochemistry·Céline ChartierJean Paul Cristol
May 22, 2013·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Evangelos TerposG David Roodman
Jul 19, 2016·Journal of Clinical Laboratory Analysis·Cecilia NapodanoUmberto Basile
Jul 13, 2017·Ocular Immunology and Inflammation·Rehan RajputPhilip I Murray

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Methods Mentioned

BETA
electrophoresis

Software Mentioned

H2SCAN
HYDRASYS 2 SCAN (

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