Performance evaluation of Abbott ARCHITECT SARS-CoV-2 IgG immunoassay in comparison with indirect immunofluorescence and virus microneutralization test

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
Silvia MeschiINMICovid-19 laboratory team

Abstract

Serological tests for anti-SARS-CoV-2 antibodies are becoming of great interest to determine seroprevalence in a given population, define previous exposure and identify highly reactive human donors for the generation of convalescent serum as therapeutic. We evaluated the diagnostic performance of the Abbott ARCHITECT SARS-CoV-2 IgG test, a fully automated indirect immunoassay that detects antibodies directed to a recombinant SARS-CoV-2 Nucleocapsid antigen. Abbott ARCHITECT SARS-CoV-2 IgG immunoassay was compared to an indirect immunofluorescence assay (IFA) on sera from patients with COVID-19 collected at different days after symptoms onset or infected by other human coronaviruses. Comparison with neutralization test was also performed. After 7, 14 and >14 days after onset ARCHITECT was positive on 8.3 %; 61.9 % and 100 % of the tested samples compared to 58.3 %; 85.7 % and 100 % by IFA. The sensitivity was 72 % vs. IFA and 66.7 % vs. a real-time PCR, the specificity was 100 %. On 18 samples with neutralizing activity, 17 were positive by Abbott ARCHITECT SARS-CoV-2 IgG. In our study, Abbott ARCHITECT SARS-CoV-2 IgG assay showed a satisfactory performance, with a very high specificity. IgG reactivity against SARSCoV-2 N antige...Continue Reading

Citations

Oct 8, 2020·Clinical Chemistry and Laboratory Medicine : CCLM·Mary Kathryn BohnUNKNOWN and the IFCC Taskforce on COVID-19
Sep 15, 2020·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Julien MarletCatherine Gaudy-Graffin
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Feb 25, 2021·JAMA Internal Medicine·Raymond A HarveyLynne T Penberthy
Feb 27, 2021·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Jan Van ElslandePieter Vermeersch
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Sep 8, 2021·Clinical Chemistry and Laboratory Medicine : CCLM·Silvia MeschiUNKNOWN INMI Covid-19 laboratory and investigation team

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Methods Mentioned

BETA
light microscopy

Software Mentioned

ARCHITECT
Excel

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