Performance evaluation of leukocyte differential on the hematology analyzer Celltac G compared with two hematology analyzers, reference flow cytometry method, and two manual methods.

Journal of Clinical Laboratory Analysis
Kenji YamadeToshinori Kamisako

Abstract

The automated hematology analyzer Celltac G (Nihon Kohden) was designed to improve leukocyte differential performance. Comparison with analyzers using different leukocyte detection principles and differential leukocyte count on wedge film (Wedge-Diff) shows its clinical utility, and comparison with immunophenotypic leukocyte differential reference method (FCM-Ref) shows its accuracy performance. For method comparison, 598 clinical samples and 46 healthy volunteer samples were selected. The two comparative hematology analyzers (CAAs) used were XN-9000 (Sysmex) and CELL-DYN Sapphire (Abbott). The FCM-Ref provided by the Japanese Society for Laboratory Hematology was selected, and a flow cytometer Navios (Beckman-Coulter) was used. In manual differential, two kinds of automated slide makers were used: SP-10 (Sysmex) for wedge technique and SPINNER-2000 (Lion-Power) for spinner technique. The spinner technique avoids the issue of Wedge-Diff smudge cells by removing the risk of breaking cells and non-uniformity of blood cell distribution on films (Spinner-Diff). The Celltac G showed sufficient comparability (r = 0.67-1.00) with the CAAs for each leukocyte differential counting value at 0.00-40.87(109 /L), and sufficient comparabilit...Continue Reading

References

Jul 1, 1974·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J W Bacus
Mar 30, 2005·American Journal of Hematology·Joanne Haller HasskampElias George Elias
Mar 29, 2014·International Journal of Laboratory Hematology·C BriggsUNKNOWN International Council for Standardization of Haematology
May 11, 2016·International Journal of Laboratory Hematology·J Y Vis, A Huisman
Aug 5, 2017·Journal of Clinical Pathology·Antonio La GioiaVincenzo Rocco

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