Abstract
Accurate platelet counting is essential for risk assessment of bleeding and thrombosis. Abbott Alinity hq hematology analyzer was recently introduced, and its performance in platelet counting has yet to be evaluated comprehensively. In this study, we evaluated the performance of the optical platelet counting of Abbott Alinity hq (Alinity-PLT) and the impedance and fluorescent platelet counting of Sysmex XN-9000 (XN-PLT-I and XN-PLT-F) compared with the international reference method. Blood samples were analyzed via Alinity hq and XN-9000 with PLT-F channel. Immuno-platelet (ImmnoPLT) reference method was performed with CD41/CD61 antibodies using FACSLyricTM flow cytometer (BD). Precision was determined using 10 replicates in a single run, and the platelet counts of Alinity-PLT, XN-PLT-I, XN-PLT-F, and ImmnoPLT were compared. At a platelet count of 13 × 109 /L, the CVs of Alinity-PLT, XN-PLT-I, and XN-PLT-F were 4.2%, 6.7%, and 4.3%, respectively, and at a platelet count of 44 × 109 /L, all showed a CV of less than 3%. For the total 210 samples, all three methods showed a very strong correlation with ImmunoPLT (r > 0.99). For platelet levels below 20 × 109 /L, XN-PLT-F showed the strongest correlation with ImmunoPLT (r = 0.975),...Continue Reading
References
Mar 13, 2001·American Journal of Clinical Pathology·P HarrisonUNKNOWN International Society of Laboratory Hematology Task Force for the Reference Platelet Count
Mar 15, 2001·American Journal of Clinical Pathology·UNKNOWN International Council for Standardization in Haematology Expert Panel on Cytometry, UNKNOWN International Society of Laboratory Hematology Task Force on Platelet Counting
Nov 16, 2011·Thrombosis·Jonathan S Bleeker, William J Hogan
Sep 21, 2013·American Journal of Clinical Pathology·Margreet SchoorlJohannes van Pelt
Nov 5, 2014·Annals of Laboratory Medicine·Sang Hyuk ParkSeongsoo Jang
Nov 11, 2014·Annals of Internal Medicine·Richard M KaufmanAaron A R Tobian
May 11, 2016·International Journal of Laboratory Hematology·J Y Vis, A Huisman
Dec 23, 2016·British Journal of Haematology·Lise J EstcourtUNKNOWN British Committee for Standards in Haematology
Sep 18, 2018·Transfusion·Kornelia HummelLudi P J M van Dun
May 28, 2019·PloS One·Yi SunChengbin Wang
Jul 5, 2019·International Journal of Laboratory Hematology·Kwang-Sook WooJin-Yeong Han
Jul 16, 2019·International Journal of Laboratory Hematology·Yolien Van der BekenKristin Jochmans
Jul 25, 2019·Clinical Chemistry and Laboratory Medicine : CCLM·Christiaan L SlimHuibert Storm