Performance of a new molecular assay for the detection of gastrointestinal pathogens

Access Microbiology
Bruce A Gingras, Jack A Maggiore

Abstract

Conventional diagnostic laboratory algorithms for determining the cause of infectious gastroenteritis include culture, biochemical identification and immunoassays. In addition, multiplex PCR-based testing has advanced into the gastroenterology diagnostic arena in recent years. The purpose of this study was to evaluate the performance of a new molecular test (Diagnostics Solutions Laboratory GI-MAP) for the detection of bacterial and parasitic pathogens in stool samples spiked with known organisms. Faeces from a healthy human subject were pooled into a standard matrix and screened for the absence of bacteria, parasites and Helicobacter pylori antigen. Once confirmed negative single faecal aliquots from the matrix were spiked with solely one pathogen-type from a panel of 14 bacterial pathogens or one of 2 parasitic pathogens at a density of 5×106 organisms ml-1. Sixteen spiked samples in appropriate transport media were sent to two testing labs, specifically a reference site using the PCR-based BioFire FilmArray Gastrointestinal Panel, and a second lab using the GI-MAP assay. Seven negative control samples comprised solely of stool matrix were also submitted. Significant variability was found when the GI-MAP assay was used to tes...Continue Reading

References

Jan 6, 2007·BMJ : British Medical Journal·Elizabeth Jane Elliott
Apr 22, 2011·Journal of Clinical Microbiology·Kenneth H RandHerbert J Houck
Jul 5, 2019·Journal of Clinical Microbiology·William StokesDylan R Pillai

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Citations

Nov 11, 2021·Expert Review of Molecular Diagnostics·Leonard SchueleNatacha Couto

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Methods Mentioned

BETA
PCR
nucleic acid amplification
bronchoalveolar lavage

Software Mentioned

MAP
GI

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