Performance of a novel test for IgM and IgG antibodies in subjects with culture-documented genital herpes simplex virus-1 or -2 infection

Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases
R Morrow, David Friedrich

Abstract

Novel tests (BioPlex) for herpes simplex virus-1 (HSV-1) and HSV-2 IgG were compared with HerpeSelect HSV-1 and HSV-2 ELISAs for type-specific IgG. The sensitivity and specificity of BioPlex HSV-1 IgG were 94% (84/89) and 96% (119/124), respectively, with unselected sera, while the sensitivity and specificity of BioPlex HSV-2 IgG were 92% (109/118) and 98% (95/97), respectively. BioPlex IgM was compared with Diamedix IgM against sera from patients with culture-documented genital herpes. The test results were concordant in 81% of sera from HSV-1 patients and in 90% of sera from HSV-2 patients. Use of BioPlex IgM in addition to BioPlex IgG tests increased HSV-2 seroconversion detection from 47% of subjects to 70%. Use of Diamedix IgM in addition to Focus IgG ELISA increased HSV-2 detection from 40% of subjects to 70%. IgM was detected by BioPlex in 63% of sera from patients with early HSV-2 infection (< 30 days) and in 59% of sera by Diamedix. IgM was also detected in a large proportion of sera from subjects with established HSV-2 infection (33% by BioPlex and 29% by Diamedix). Addition of IgM testing substantially increased the ability to detect seroconversion early in infection. IgM is an indicator of recent infection only in s...Continue Reading

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Citations

Sep 10, 2014·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Enda Shevlin, Rhoda Ashley Morrow
Oct 18, 2013·Journal of Clinical Microbiology·Neil W AndersonNathan A Ledeboer
Aug 14, 2010·Journal of Virological Methods·Hua YanMingchun Ji
Aug 23, 2008·Autoimmunity Reviews·Ravi KaulGreg Marr
Jun 6, 2007·Journal of Virological Methods·Andreas Sauerbrei, Peter Wutzler
Dec 29, 2010·The American Journal of Medicine·Jesse J KellerAndrea Kalus
Jan 15, 2014·Journal of Virological Methods·Kristin LiermannAndreas Sauerbrei
Mar 3, 2011·International Journal of STD & AIDS·R PatelUNKNOWN IUSTI/WHO Europe
Feb 11, 2018·American Journal of Reproductive Immunology : AJRI·Fabiana Finger-JardimMarcelo Alves Soares
Aug 10, 2010·Sexually Transmitted Diseases·George Zahariadis, Alberto Severini
May 24, 2020·The Journal of Applied Laboratory Medicine·Sarah Jung, Elitza S Theel
Jun 24, 2016·Microbiology Spectrum·Myron J LevinD Scott Schmid
Jul 23, 2021·MMWR. Recommendations and Reports : Morbidity and Mortality Weekly Report. Recommendations and Reports·Kimberly A WorkowskiGail A Bolan

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