Performance of Biochip system in detecting drug resistant and multidrug-resistant tuberculosis using sputum collected from multiple clinical settings in Zhejiang, China

Scientific Reports
Kaijin XuLanjuan Li

Abstract

The objective of the present study was to conduct a multicentre, prospective evaluation of the diagnostic performance of the Biochip system for the detection of drug-resistant tuberculosis using smear-positive sputum specimens. This prospective study evaluated the diagnostic performance of this new platform for drug resistant and multidrug-resistant tuberculosis (MDR-TB) using 1491 smear-positive sputum specimens collected from multiple clinical settings. Using conventional culture-based culturing and drug-susceptibility testing as reference standards, the biochip system had a sensitivity of 86.08% and a specificity of 97.7% for rifampicin (RIF) detection, in detecting isoniazid (INH) resistance, it had a sensitivity of 79.36% and a specificity of 98.71%. With respect to MDR-TB detection, the sensitivity was 78.01% and the specificity was 98.86%. The performance only varies among different sites for RIF resistance, and there are no other statistically difference in diagnostic performance for other variables considered. The Biochip system shows favourable sensitivity and specificity for RIF and INH resistance, along with MDR-TB detection, directly using clinical smear-positive sputum samples. It is an alternative to conventional...Continue Reading

References

Nov 7, 2006·Lancet·Annelies Van Rie, Donald Enarson
Aug 8, 2009·Respiratory Medicine·Suhail Ahmad, Eiman Mokaddas
Mar 12, 2011·The Journal of Antimicrobial Chemotherapy·Gilman Kit Hang SiuWing-Cheong Yam
Jun 8, 2012·The New England Journal of Medicine·Yanlin ZhaoYu Wang
Jun 13, 2012·The International Journal of Tuberculosis and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·F DrobniewskiD Papaventsis
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Mar 22, 2013·Journal of Clinical Microbiology·Yu PangYanlin Zhao
Dec 3, 2014·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Xichao OuYanlin Zhao

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Methods Mentioned

BETA
PCR
chips
chip
PCRs

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