Performance of commercial reverse line blot assays for human papillomavirus genotyping.

Journal of Clinical Microbiology
Martin SteinauTiequn Zhou

Abstract

The performance of three line blot assays (LBAs), the Linear Array HPV genotyping assay (LA) (Roche Diagnostics), INNO-LiPA HPV Genotyping Extra (LiPA) (Innogenetics), and the reverse hybridization assay (RH) (Qiagen), was evaluated using quantitated whole genomic human papillomavirus (HPV) plasmids (types 6, 11, 16, 18, 31, 33, 35, 39, 51, 52, 56, 58, 59, and 68b) as well as epidemiologic samples. In a plasmid titration series, LiPA and RH did not detect 50 international units (IU) of HPV type 18 (HPV18) in the presence of 5 × 10(4) IU or more of HPV16. HPV DNA (1 to 6 types) in the plasmid challenges at 50 IU or genome equivalents (GE) were identified with an accuracy of 99.9% by LA, 97.3% by LiPA, and 95.4% by RH, with positive reproducibility of 99.8% (kappa = 0.992), 88.2% (kappa = 0.928), and 88.1% (kappa = 0.926), respectively. Two instances of mistyping occurred with LiPA. Of the 120 epidemiologic samples, 76 were positive for high-risk types by LA, 90 by LiPA, and 69 by RH, with a positive reproducibility of 87.3% (kappa = 0.925), 83.9% (kappa = 0.899), and 90.2% (kappa = 0.942), respectively. Although the assays had good concordance in the clinical samples, the greater accuracy and specificity in the plasmid panel sug...Continue Reading

References

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Citations

Apr 23, 2014·Emerging Infectious Diseases·Martin SteinauUNKNOWN HPV Typing of Cancers Workgroup
Jun 3, 2014·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Vikrant V SahasrabuddheNicolas Wentzensen
Nov 28, 2012·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Frédérique MoreauPatrick Soussan
Jan 19, 2016·Infectious Agents and Cancer·Samira Zoa AssoumouMy Mustapha Ennaji
Nov 26, 2015·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Marc T GoodmanElizabeth R Unger
Feb 13, 2013·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Catia SiasMaria Rosaria Capobianchi
Dec 4, 2014·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Paola AlberizziEnrico Maria Silini
Aug 11, 2012·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Luisa BarzonGiorgio Palù
Nov 9, 2016·The Journal of Infectious Diseases·Abbey B BerensonMahbubur Rahman
Apr 6, 2019·Current Molecular Medicine·Abhilasha GautamJaya Chakravarty
Apr 24, 2019·Diagnostic Pathology·Cristina Artaza-IrigarayAdriana Aguilar-Lemarroy

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