Performance of Commercially Available Serological Screening Tests for Human T-Cell Lymphotropic Virus Infection in Brazil

Journal of Clinical Microbiology
Vanessa da Silva BritoBernardo Galvão-Castro

Abstract

Serological screening for human T-cell lymphotropic virus type 1 (HTLV-1) is usually performed using enzyme-linked immunosorbent assay (ELISA), particle agglutination, or chemiluminescence assay kits. Due to an antigen matrix improvement entailing the use of new HTLV antigens and changes in the format of HTLV screening tests, as well as newly introduced chemiluminescence assays (CLIAs), a systematic evaluation of the accuracy of currently available commercial tests is warranted. We aimed to assess the performance of commercially available screening tests for HTLV infection diagnosis. A diagnostic accuracy study was conducted on a panel of 397 plasma samples: 200 HTLV-negative plasma samples, 170 HTLV-positive plasma samples, and 27 plasma samples indeterminate by Western blotting (WB). WB-indeterminate samples (i.e., those yielding no specific bands for HTLV-1 and/or HTLV-2) were assessed by PCR, and the results were used to compare agreement among the commercially available ELISA screening tests. For performance analysis, WB-indeterminate samples were excluded, resulting in a final study panel of 370 samples. Three ELISA kits (Murex HTLV-1/2 [Murex], anti-HTLV-1/2 SYM Solution [SYM Solution], and Gold ELISA HTLV-1/2 [Gold ELIS...Continue Reading

References

Aug 15, 1992·American Journal of Ophthalmology·M MochizukiN Miyata
May 3, 1986·Lancet·M OsameM Tara
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·B J PoieszR C Gallo
Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M YoshidaY Hinuma
Jan 1, 1995·The American Journal of Medicine·V SorianoJ González-Lahoz
Jul 1, 1997·Japanese Journal of Cancer Research : Gann·I MboudjekaM Hayami
Nov 15, 2003·Journal of Clinical Epidemiology·Afina S GlasPatrick M M Bossuyt
Jul 6, 2004·Annals of Neurology·Abelardo Araujo, William W Hall
May 11, 2005·Retrovirology·Sara CalattiniAntoine Gessain
May 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Nathan D WolfeWilliam M Switzer
Nov 6, 2007·Journal of Virological Methods·Carolina A BeriniMirna M Biglione
Dec 25, 2007·Revista Do Instituto De Medicina Tropical De São Paulo·Fabrício JacobAdele Caterino-de-Araujo
Jan 22, 2008·Journal of Medical Virology·Xiaoxing QiuSushil G Devare
Nov 11, 2008·Journal of Virological Methods·Adele Caterino-de-Araujo
Mar 17, 2010·Diagnostic Microbiology and Infectious Disease·Hans-Peter KapprellX Qiu
Jul 22, 2010·Journal of Medical Virology·Kerstin MalmSören Andersson
Feb 26, 2011·Journal of Virological Methods·Emanuela A S CostaAdele Caterino-de-Araujo
Apr 5, 2011·The Journal of Infectious Diseases·Philippe MauclèreAntoine Gessain
Aug 16, 2011·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Houshang RafatpanahAli Bazarbachi
Aug 27, 2011·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Cristina Castro-Lima VargensBernardo Galvão-Castro
Nov 20, 2012·Frontiers in Microbiology·Antoine Gessain, Olivier Cassar
Feb 5, 2015·Revista Do Instituto De Medicina Tropical De São Paulo·Arthur Paiva, Jorge Casseb
Oct 30, 2015·BMJ : British Medical Journal·Patrick M BossuytUNKNOWN STARD Group
Mar 9, 2017·PLoS Neglected Tropical Diseases·Fred Luciano Neves SantosYara de Miranda Gomes
Mar 28, 2017·The Brazilian Journal of Infectious Diseases : an Official Publication of the Brazilian Society of Infectious Diseases·Karoline Rodrigues CamposAdele Caterino-de-Araujo

❮ Previous
Next ❯

Citations

Jun 26, 2020·Frontiers in Microbiology·Huimin JiLunan Wang
Oct 11, 2019·Journal of Clinical Microbiology·Timothy WooGraham P Taylor
Sep 13, 2019·PLoS Neglected Tropical Diseases·Carolina RosadasGraham P Taylor
Feb 13, 2020·Revista Da Sociedade Brasileira De Medicina Tropical·Adele Caterino-de-AraujoRegina Célia Moreira
Oct 17, 2019·Revista Da Sociedade Brasileira De Medicina Tropical·Marzia Puccioni-SohlerThessika Hialla Almeida Araújo
Oct 20, 2020·The Journal of Clinical Investigation·Kenta TezukaIsao Hamaguchi
Mar 9, 2021·Revista Da Sociedade Brasileira De Medicina Tropical·Carolina RosadasGraham Philip Taylor
Jun 10, 2021·Revista Da Sociedade Brasileira De Medicina Tropical·Adele Caterino-de-AraujoCarolina Rosadas
Sep 10, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Carolina de Alcantara ManeschyCarlos Eduardo de Melo Amaral

❮ Previous
Next ❯

Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA
PCR
enzyme-linked
density gradient centrifugation
electrophoresis
Assay
ELISAs

Software Mentioned

Prism
Murex
GraphPad

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.