Performance of Genotype MTBDRsl V2.0 over the Genotype MTBDRsl V1 for detection of second line drug resistance: An Indian perspective

PloS One
Syed Beenish RufaiSarman Singh

Abstract

Genotype MTBDRsl Version 1 (V1.0) was recommended as an initial test for rapid detection of pre-extensively drug resistant (pre-XDR) and extensively drug resistant tuberculosis (XDR-TB). However, in recent years a number of novel mutations are identified that confer resistance. Thus, Genotype MTBDRsl V2.0 was endorsed by WHO. Though, Genotype MTBDRsl V2.0 has been rolled out in national TB programme in 2018, there is dearth of data from India on its performance for second line drug susceptibility testing (DST). For this, performance of new version was evaluated on 113 MDR-TB isolates. The results showed that 39 (34.5%) of these isolates were resistant to FQ and 7 (6.2%) were XDR by Genotype MTBDRsl V2.0. Amongst the FQ resistant isolates most prevalent mutation was ΔWT3-D94G (17; 38.6%) and N538D (12; 85.7%). Among the AG/CP and KAN resistant isolates most common mutation in the rrs region was ΔWT1-A1401G (5; 71.4%) and C-14T (2; 28.5%) in eis gene. Second line Bactec MGIT-960 detected 40 (35.4%) isolates as resistant to FQ and 6 (5.3%) as XDR isolates, whereas Genotype MTBDRsl V1.0 also detected 39 (34.5%) as resistant to FQ but missed 2 isolates in correctly identifying as XDR (5; 4.4%). Thus, concordance of second line Bacte...Continue Reading

References

Apr 21, 2009·Lancet·Abigail WrightUNKNOWN Global Project on Anti-Tuberculosis Drug Resistance Surveillance
Nov 13, 2009·Proceedings of the National Academy of Sciences of the United States of America·M Analise ZaunbrecherJames E Posey
Feb 27, 2010·The European Respiratory Journal·V LeimaneT H Holtz
May 28, 2010·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Karen R JacobsonMegan B Murray
Nov 20, 2012·The Journal of Molecular Diagnostics : JMD·Jialin JinWenhong Zhang
Apr 23, 2013·Tuberculosis Research and Treatment·Hyejin KimSungweon Ryoo
May 28, 2013·BMC Infectious Diseases·Danila V ZimenkovDmitry A Gryadunov
Jan 17, 2014·The International Journal of Tuberculosis and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·N TukvadzeR R Kempker
Feb 26, 2016·American Journal of Respiratory and Critical Care Medicine·Maha R FarhatMegan Murray
Nov 1, 2016·Antimicrobial Agents and Chemotherapy·Suporn PholwatEric Houpt
Mar 23, 2018·The International Journal of Tuberculosis and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·R YadavS Sethi
Feb 26, 2019·Microbiology Resource Announcements·Syed Beenish Rufai, Sarman Singh

❮ Previous
Next ❯

Methods Mentioned

BETA
lavage
biopsy
PCR
nucleic acid amplification

Software Mentioned

BD Epicenter
OpenEpi

Related Concepts

Related Feeds

Birth Defects

Birth defects encompass structural and functional alterations that occur during embryonic or fetal development and are present since birth. The cause may be genetic, environmental or unknown and can result in physical and/or mental impairment. Here is the latest research on birth defects.

Addiction

This feed focuses mechanisms underlying addiction and addictive behaviour including heroin and opium dependence, alcohol intoxication, gambling, and tobacco addiction.

Related Papers

Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy
Young Seok LeeTae Sun Shim
The International Journal of Tuberculosis and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease
R J ChandakJ Tiwari
© 2021 Meta ULC. All rights reserved