Performance of light-emitting diode fluorescence microscope for diagnosis of tuberculosis

International Journal of Mycobacteriology
Manpreet BhallaRohit Sarin

Abstract

Fluorescence microscopy (FM) over the years has shown the potential for increasing the performance of microscopy. The present study was aimed to access the performance of the LED microscope for the detection of acid fast bacilli in a tuberculosis (TB) endemic country. The study was conducted at a National Reference Laboratory (NRL) in New Delhi, India. Sputum samples were collected from suspected TB patients. Each sample was processed with Auramine O and ZN methods. Auramine O stained smears were evaluated using two different excitatory light sources (MVP and LED); and ZN stained smears were examined under light microscope. The mean time required to read the smears with different modalities was recorded. Bacterial cultures provided the reference standard. A total of 200 patients were included in this study. Sensitivity and specificity for the LED assessment, MVP assessment and light microscopy were 83.1% and 82.4%, 78.5% and 87.5% and 81.6% and 83.5%, respectively. Mean reading time was approximately three times faster than ZN microscopy. The mean time to read a negative smear was 2min with fluorescence microscopy and 5min with light microscopy with time savings of 60%. Although the use of LED-FM only marginally increased sensi...Continue Reading

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Citations

Dec 15, 2015·International Journal of Mycobacteriology·Ritu Singhal, Vithal Prasad Myneedu
Aug 5, 2017·International Journal of Mycobacteriology·Prasanna BhirudAbhay Chowdhary
Dec 10, 2015·The European Respiratory Journal·Eva W ChangMaryline Bonnet
Sep 25, 2019·Nature Reviews. Urology·Asif MuneerAlimuddin Zumla
Jul 18, 2018·Tuberculosis Research and Treatment·Yassir A ShuaibKatharina Kranzer
Jan 5, 2020·Lasers in Medical Science·Anushka OjhaNirmal Mazumder
Dec 10, 2019·The Indian Journal of Tuberculosis·Sangeeta SharmaV P Myneedu
Mar 11, 2021·Expert Review of Anti-infective Therapy·Ekta Kamra, Promod K Mehta
Mar 25, 2017·Microbiology Spectrum·Catherine Vilchèze, Laurent Kremer

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