Performance of optoacoustic and fluorescence imaging in detecting deep-seated fluorescent agents

Biomedical Optics Express
Zhenyue ChenDaniel Razansky

Abstract

Fluorescent contrast agents are widely employed in biomedical research. While many studies have reported deep tissue imaging of fluorescent moieties using either fluorescence-based or absorption-based (optoacoustic) imaging systems, no systematic comparison has been performed regarding the actual performance of these imaging modalities in detecting deep-seated fluorescent agents. Herein, an integrated imager combining epi-fluorescence and volumetric optoacoustic imaging capabilities has been employed in order to evaluate image degradation with depth for several commonly-used near-infrared dyes in both modes. We performed controlled experiments in tissue-mimicking phantoms containing deeply embedded targets filled with different concentrations of Alexa Fluor 700, Alexa Fluor 750, indocyanine green (ICG) and IRDye 800CW. The results are further corroborated by multi-modal imaging of ICG through mouse tissues in vivo. It is shown that optoacoustics consistently provides better sensitivity in differentiating fluorescent targets located at depths beyond 2 mm in turbid tissues, as quantified by evaluating image contrast, signal to noise ratio and spatial resolution performance.

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Citations

Mar 11, 2019·Journal of Fluorescence·V V MaslovI M Pritula
Dec 12, 2019·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Damien E DobsonHans F Schmitthenner
Dec 5, 2018·Journal of Biophotonics·Vijitha PeriyasamyManojit Pramanik
Sep 15, 2020·Light, Science & Applications·Zhenyue ChenDaniel Razansky
Jul 6, 2021·Biomedical Optics Express·Maksim D MokrousovDmitry A Gorin
Aug 20, 2021·EMBO Molecular Medicine·Shan HuangVasilis Ntziachristos
Sep 1, 2021·Light, Science & Applications·Zhenyue ChenDaniel Razansky

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