Performance of the Accelerate Pheno™ system for identification and antimicrobial susceptibility testing of a panel of multidrug-resistant Gram-negative bacilli directly from positive blood cultures

The Journal of Antimicrobial Chemotherapy
Alix PantelJean-Philippe Lavigne

Abstract

To evaluate the performance of the Accelerate Pheno™ system for the identification and antimicrobial susceptibility testing (AST) of a panel of Gram-negative bacilli (GNB) with different resistance profiles (e.g. penicillinases, ESBLs, cephalosporinase overproduction, carbapenemases, impermeability) directly from positive blood cultures in <7 h. A panel of 105 clinical strains previously characterized for the presence of β-lactamase-encoding genes was tested. Approximately 100 cfu of each isolate was inoculated into sterile blood culture bottles and incubated in a BD BACTEC™ FX automated system (Becton Dickinson, USA). Positive blood cultures were subjected to parallel testing using the Accelerate Pheno™ system and conventional culture methods [identification of isolated colonies by MALDI-TOF and VITEK® 2 system (bioMérieux, France), and AST by disc diffusion and Etest following EUCAST recommendations]. The overall identification agreement between the Accelerate Pheno™ system and conventional culture methods was 100% (105/105). The overall categorical agreement between the system and culture-based AST was 94.9% (1169/1232), with rates for minor errors of 4.1% (51/1232), major errors 0.3% (4/1232) and very major errors 0.7% (8/1...Continue Reading

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Citations

Jul 6, 2018·Microbial Genomics·Wim L CuypersSandra Van Puyvelde
Jan 15, 2019·Expert Review of Molecular Diagnostics·Grégory DubourgFlorence Fenollar
Aug 23, 2019·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Gabriele BiancoCristina Costa
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Oct 29, 2021·Expert Review of Anti-infective Therapy·Vanesa Anton-Vazquez, Timothy Planche

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