Performance of the flow cytometric E-screen assay in screening estrogenicity of pure compounds and environmental samples

The Science of the Total Environment
C VanparysW De Coen

Abstract

In vitro estrogenicity screens are believed to provide a first prioritization step in hazard characterization of endocrine disrupting chemicals. When applied to complex environmental matrices or mixture samples, they have been indicated valuable in estimating the overall estrogen-mimicking load. In this study, the performance of an adapted format of the classical E-screen or MCF-7 cell proliferation assay was profoundly evaluated to rank pure compounds as well as influents and effluents of sewage treatment plants (STPs) according to estrogenic activity. In this adapted format, flow cytometric cell cycle analysis was used to allow evaluation of the MCF-7 cell proliferative effects after only 24 h of exposure. With an average EC(50) value of 2 pM and CV of 22%, this assay appears as a sensitive and reproducible system for evaluation of estrogenic activity. Moreover, estrogenic responses of 17 pure compounds corresponded well, qualitatively and quantitatively, with other in vitro and in vivo estrogenicity screens, such as the classical E-screen (R(2)=0.98), the estrogen receptor (ER) binding (R(2)=0.84) and the ER transcription activation assay (R(2)=0.87). To evaluate the applicability of this assay for complex samples, influents...Continue Reading

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Citations

Mar 17, 2016·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Boris V KrivoshievSteven J Husson
Jun 14, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Zhengyan LiJiangling Li
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Apr 15, 2014·The Science of the Total Environment·Alfredo ParrellaMarina Isidori
May 21, 2016·Environmental Science and Pollution Research International·Sanhua XiaoFei Tang
Sep 18, 2016·Basic & Clinical Pharmacology & Toxicology·Cristina LeminiAlejandro Jiménez-Orozco
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Mar 30, 2018·Water Science and Technology : a Journal of the International Association on Water Pollution Research·Jian Lin ChenNaresh Singhal
Jul 3, 2021·International Journal of Environmental Research and Public Health·Rehab ElfadulAli Ishaque

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