Performance of the Neutral Red uptake assay in the COLIPA international validation study on alternatives to the rabbit eye irritation test

Toxicology in Vitro : an International Journal Published in Association with BIBRA
P A JonesH Kojima

Abstract

The neutral red uptake (NRU) assay was included as part of the COLIPA international validation trial of in vitro alternatives to the Draize eye irritation test. In a blind trial, 55 substances were tested at four laboratories. Following testing, a prediction of the in vivo Draize modified maximum average score (MMAS) for each substance was made by each laboratory using a prediction model relating mean NR(50) value (concentration causing 50% reduction in NRU from that of untreated control cells) to MMAS. Following statistical analysis of the results and breaking of the code, assessment of the results and further analysis was carried out by the participating laboratories. This paper presents the conclusions with regard to the NRU assay. The initial trial analysis indicated that the interlaboratory reproducibility of results of the NRU assay was good. However, there was a poor correlation between observed and predicted MMAS (using the proposed prediction model) when all the test substances were analysed together (r=0.246). Data analysis of subsets of substances indicated that the best predictions were for pure surfactants only (r=0.843) although this data did not fit within the limits of the prediction model. The NRU assay therefo...Continue Reading

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Citations

Sep 21, 2000·Toxicology Letters·P da Silva MeloM Haun
Nov 6, 2003·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A V King, P A Jones
Sep 5, 2012·The Journal of Pharmacy and Pharmacology·Narinthorn KhositsuntiwongJiradej Manosroi
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Oct 1, 2005·Alternatives to Laboratory Animals : ATLA·Chantra EskesValérie Zuang
Feb 6, 2021·In Vitro Cellular & Developmental Biology. Animal·Yulia Kaluzhny, Mitchell Klausner

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