Performance of the Pollen Tube Growth Test in the COLIPA Validation Study on Alternatives to the Rabbit Eye Irritation Test

Toxicology in Vitro : an International Journal Published in Association with BIBRA
Udo KristenR Schell

Abstract

In the present paper, we describe and analyse the performance and the results of the pollen tube growth (PTG) test applied to the COLIPA international validation study of in vitro alternatives to the Draize eye irritation test. The PTG test, based on photometric quantification of in vitro pollen tube mass production, was used by three independent laboratories to estimate the acute eye irritation potentials of 23 ingredients and 32 cosmetic formulations. Basing on historical Draize test data and on IC(50) values of previously tested cosmetic formulations, a mathematical formula was generated to predict rabbit eye irritation potentials from PTG test results. Statistical evaluation of the calculated modified maximal average scores (MMAS) revealed a high prediction capability of the PTG test in regard to the finished formulations but a relatively low one for alcohols, higher concentrated cationic surfactants, and acidic and alkaline materials. Furthermore, our results indicated that the PTG test was able to produce precise IC(50) values without any limitations from all of the 55 test substances with good intra- and interlaboratory reproducibility. From these findings we suggest that the PTG test is not a validated test at present b...Continue Reading

References

Oct 1, 1994·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·S D GettingsR Gay
Jun 1, 1994·Cell Biology and Toxicology·F A BarileU Kristen
Jan 1, 1996·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·S D GettingsJ Zyracki
Feb 1, 1997·Toxicology in Vitro : an International Journal Published in Association with BIBRA·P G BrantomM Stern
Feb 1, 1997·Toxicology in Vitro : an International Journal Published in Association with BIBRA·U Kristen

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Citations

Mar 1, 2000·Mycotoxin Research·K SenkpielH Ohgke
Nov 15, 2008·Environmental Science and Pollution Research International·K JungG Schüürmann
May 25, 2006·Brazilian Dental Journal·Udo Kristen, Reinhard E Friedrich
Dec 17, 2008·Alternatives to Laboratory Animals : ATLA·Christina GrindonJohn F Garrod
Oct 1, 2005·Alternatives to Laboratory Animals : ATLA·Chantra EskesValérie Zuang
Mar 13, 2008·Alternatives to Laboratory Animals : ATLA·Christina GrindonJohn F Garrod

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