Abstract
The PROTIA™ Allergy-Q® enzyme immunoassay (EIA) is a recently developed screening assay for specific immunoglobulin E (sIgE) for multiple allergens. The ImmunoCAP® fluorescent EIA (FEIA) system is the most widely used method for sIgE detection. In this study, we evaluated the performance of the Allergy-Q® system compared to the ImmunoCAP® system. We compared the 2 systems using sera from 260 Korean allergy patients suffering from asthma (26.5%), allergic rhinitis (42.3%), atopic dermatitis (67.7%), and food allergy (18.1%). We compared sIgE-measurement results for 7 inhalant allergens, 5 food allergens, and 4 microorganism allergens. Overall, 1,799 paired assay results were analyzed. Except mugwort and alternaria, most of the allergen-sIgE results showed intra-class correlation coefficients of >0.5. Inter-assay class associations were reliable for most allergens (gamma=0.858-0.987, P<0.001). Passing-Bablok regression analysis showed multiple differences in intercept and slope. The inter-method concordance was moderate to substantial for most allergens (κ=0.713-0.898, P<0.001). The PROTIA™ Allergy-Q® EIA system exhibited good detection performance compared to the ImmunoCAP® FEIA system in Korean allergic patients. However, becau...Continue Reading
References
Nov 1, 1985·The Journal of Allergy and Clinical Immunology·M C SwansonC E Reed
Nov 25, 1967·Lancet·L WideS G Johansson
Jan 1, 1984·Critical Reviews in Clinical Laboratory Sciences·R G Hamilton, N F Adkinson
Jun 5, 1998·Scandinavian Journal of Primary Health Care·M J CrobachJ D Mulder
Dec 22, 1999·Allergy·D GislasonG Boman
May 10, 2001·The Journal of Allergy and Clinical Immunology·H A Sampson
Jan 1, 2004·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·P Brock Williams
May 21, 2005·Clinical Chemistry·Markus OllertJohannes Ring
Feb 14, 2007·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Geunwoong NohJae-Won Oh
Dec 6, 2008·Clinica Chimica Acta; International Journal of Clinical Chemistry·Yong Won LeeJung-Won Park
Mar 6, 2009·Clinica Chimica Acta; International Journal of Clinical Chemistry·Sungsil LeeHyon Suk Kim
Nov 7, 2009·The Korean journal of laboratory medicine·Woo Ri JangJin Ju Kim
Apr 2, 2010·Allergy, Asthma & Immunology Research·Jae Woo JungByoung Whui Choi
Nov 3, 2010·Allergy·T ZuberbierJ Bousquet
Dec 3, 2010·The Journal of Allergy and Clinical Immunology·Linda CoxDana Wallace
Jan 11, 2011·Allergy, Asthma & Immunology Research·Nami Shrestha PalikheHae-Sim Park
Mar 8, 2012·Clinica Chimica Acta; International Journal of Clinical Chemistry·Jae-Hyun LeeJung-Won Park
Apr 27, 2012·Experimental & Applied Acarology·Kyoung Yong JeongJung-Won Park
Oct 18, 2012·Acta Dermato-venereologica·Andreas SonessonOve Bäck
Sep 5, 2013·Allergy, Asthma & Immunology Research·Hyun Jung JinHae-Sim Park
Nov 2, 2013·Allergy, Asthma & Immunology Research·Kyoung Yong JeongJung-Won Park
Nov 8, 2014·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A P UyttebroekD G Ebo
Citations
Oct 27, 2017·Annals of Laboratory Medicine·Kyung Hee ParkSang Chul Lee
Mar 27, 2019·Allergy, Asthma & Immunology Research·Sung Ryeol KimJung Won Park
May 26, 2017·Yonsei Medical Journal·Kyung Hee ParkJung Won Park
Jun 13, 2019·International Archives of Allergy and Immunology·Samuel RoethlisbergerFrançois Spertini