Performances and Reliability of Bruker Microflex LT and VITEK MS MALDI-TOF Mass Spectrometry Systems for the Identification of Clinical Microorganisms

BioMed Research International
Kivanc BilecenYahya Rauf Laleli

Abstract

In clinical microbiology laboratories, routine microbial identification is mostly performed using culture based methodologies requiring 24 to 72 hours from culturing to identification. Matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) technology has been established as a cost effective, reliable, and faster alternative identification platform. In this study, we evaluated the reliability of the two available MALDI-TOF MS systems for their routine clinical level identification accuracy and efficiency in a clinical microbiology laboratory setting. A total of 1,341 routine phenotypically identified clinical bacterial and fungal isolates were selected and simultaneously analyzed using VITEK MS (bioMérieux, France) and Microflex LT (Bruker Diagnostics, Germany) MALDI-TOF MS systems. For any isolate that could not be identified with either of the systems and for any discordant result, 16S rDNA gene or ITS1/ITS2 sequencing was used. VITEK MS and Microflex LT correctly identified 1,303 (97.17%) and 1,298 (96.79%) isolates to the species level, respectively. In 114 (8.50%) isolates initial phenotypic identification was inaccurate. Both systems showed a similar identification efficiency and workfl...Continue Reading

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Citations

Jan 25, 2017·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·W-T FanB Gu
Aug 30, 2017·Frontiers in Microbiology·Gabriella B N AssisHenrique C P Figueiredo
Jan 31, 2017·Veterinary Dermatology·Luca GuardabassiUNKNOWN ESCMID Study Group for Veterinary Microbiology
May 31, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Sachio TsuchidaFumio Nomura

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Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

VITEK
VITEK MS IVD
Bruker
MYLA
MALDI Biotyper Realtime Classification and Biotyper

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