Perfusion single photon emission computed tomography in a mouse model of neurofibromatosis type 1: towards a biomarker of neurologic deficits

Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism
Ivayla ApostolovaRalph Buchert

Abstract

Neurofibromatosis type 1 (NF1) is a single-gene disorder affecting neurologic function in humans. The NF1+/- mouse model with germline mutation of the NF1 gene presents with deficits in learning, attention, and motor coordination, very similar to NF1 patients. The present study performed brain perfusion single-photon emission computed tomography (SPECT) in NF1+/- mice to identify possible perfusion differences as surrogate marker for altered cerebral activity in NF1. Cerebral perfusion was measured with hexamethyl-propyleneamine oxime (HMPAO) SPECT in NF1+/- mice and their wild-type littermates longitudinally at juvenile age and at young adulthood. Histology and immunohistochemistry were performed to test for structural changes. There was increased HMPAO uptake in NF1 mice in the amygdala at juvenile age, which reduced to normal levels at young adulthood. There was no genotype effect on thalamic HMPAO uptake, which was confirmed by ex vivo measurements of F-18-fluorodeoxyglucose uptake in the thalamus. Morphologic analyses showed no major structural abnormalities. However, there was some evidence of increased density of microglial somata in the amygdala of NF1-deficient mice. In conclusion, there is evidence of increased perfus...Continue Reading

References

Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M KadekaroL Sokoloff
Dec 1, 1994·Journal of Neurology, Neurosurgery, and Psychiatry·P BalestriF Fazio
Jul 1, 1994·Nature Genetics·T JacksR A Weinberg
Oct 1, 1996·Seminars in Cancer Biology·K CichowskiT Jacks
Jan 16, 1998·Journal of Child Neurology·A M KaplanE M Reiman
Mar 22, 2002·Neuron·Edwin J Weeber, J David Sweatt
Jun 26, 2002·Neoplasia : an International Journal for Oncology Research·David H Gutmann, Marco Giovannini
Oct 31, 2002·Journal of Child Neurology·Rui M Costa, Alcino J Silva
Jan 14, 2003·Trends in Molecular Medicine·Rui M Costa, Alcino J Silva
Feb 14, 2003·Current Neurology and Neuroscience Reports·Tena L Rosser, Roger J Packer
Jun 6, 2003·European Journal of Paediatric Neurology : EJPN : Official Journal of the European Paediatric Neurology Society·Michael V Johnston
Feb 15, 2007·Journal of Neurology, Neurosurgery, and Psychiatry·Shelley L HymanKathryn N North
Dec 1, 2007·Nuclear Medicine Communications·Ralph BuchertVictor F Mautner
Oct 22, 2008·Journal of Medical Genetics·T TuckerV-F Mautner
Oct 12, 2010·The Cerebellum·Lianne C KrabJosef N van der Geest
Apr 26, 2011·Alzheimer's & Dementia : the Journal of the Alzheimer's Association·Clifford R JackCreighton H Phelps
May 24, 2011·International Journal of Molecular Imaging·Ciara M FinucaneStephen J Mather
Jun 10, 2011·Communicative & Integrative Biology·Marie-Ève Tremblay, Ania K Majewska
Aug 4, 2011·Brain : a Journal of Neurology·Katya RascovskyBruce L Miller
Sep 14, 2012·NeuroImage·Ivayla ApostolovaRalph Buchert
Nov 12, 2013·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Catharina LangeRalph Buchert
Dec 21, 2013·American Journal of Medical Genetics. Part a·Jonathan M PayneKathryn N North
Jan 7, 2014·Neural Plasticity·Zuzana Šišková, Marie-Ève Tremblay
Jan 18, 2014·Nature Reviews. Neuroscience·Leonie Welberg
Sep 23, 2014·Nature Neuroscience·Andrei I MoloshAnantha Shekhar

❮ Previous
Next ❯

Citations

Sep 4, 2015·Brain Research·Ivayla ApostolovaRalph Buchert
May 8, 2021·Pediatric Neurology·Matsanga Leyila KasekaNomazulu Dlamini

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

ARRIVE
HiSPECT multi - pinhole software ( SCIVIS )
SPSS Statistics
SPM
Statistical Parametric Mapping software package SPM8

Related Concepts

Related Feeds

Amygdala and Midbrain Dopamine

The midbrain dopamine system is widely studied for its involvement in emotional and motivational behavior. Some of these neurons receive information from the amygdala and project throughout the cortex. When the circuit and transmission of dopamine is disrupted symptoms may present. Here is the latest research on the amygdala and midbrain dopamine.

Amygdala: Sensory Processes

Amygdalae, nuclei clusters located in the temporal lobe of the brain, play a role in memory, emotional responses, and decision-making. Here is the latest research on sensory processes in the amygdala.