Pericentromere clustering in Tradescantia section Rhoeo involves self-associations of AT- and GC-rich heterochromatin fractions, is developmentally regulated, and increases during differentiation.

Chromosoma
Hieronim GolczykAnna Uchman-Książek

Abstract

A spectacular but poorly recognized nuclear repatterning is the association of heterochromatic domains during interphase. Using base-specific fluorescence and extended-depth-of-focus imaging, we show that the association of heterochromatic pericentromeres composed of AT- and GC-rich chromatin occurs on a large scale in cycling meiotic and somatic cells and during development in ring- and bivalent-forming Tradescantia spathacea (section Rhoeo) varieties. The mean number of pericentromere AT-rich domains per root meristem nucleus was ca. half the expected diploid number in both varieties, suggesting chromosome pairing via (peri)centromeric regions. Indeed, regular pairing of AT-rich domains was observed. The AT- and GC-rich associations in differentiated cells contributed to a significant reduction of the mean number of the corresponding foci per nucleus in relation to root meristem. Within the first 10 mm of the root, the pericentromere attraction was in progress, as if it was an active process and involved both AT- and GC-rich associations. Complying with Rabl arrangement, the pericentromeres preferentially located on one nuclear pole, clustered into diverse configurations. Among them, a strikingly regular one with 5-7 ring-arr...Continue Reading

References

Feb 1, 1980·Human Genetics·D E Comings
Jan 1, 1994·Methods in Molecular Biology·D Schweizer, P F Ambros
Jan 1, 1995·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·A HoubenM Schmid
Oct 8, 1998·The Journal of Cell Biology·A K Csink, S Henikoff
Dec 31, 2002·International Review of Cytology·Josef Loidl
Feb 25, 2005·Genome Génome / Conseil National De Recherches Canada·Hieronim GolczykAndrzej J Joachimiak
Jun 9, 2005·The Journal of Cell Biology·Alessandro BreroM Cristina Cardoso
Sep 27, 2005·Experimental Cell Research·Rémi TerranovaAmanda G Fisher
Jan 19, 2007·Nature Reviews. Genetics·Christian LanctôtThomas Cremer
Aug 28, 2007·Genetics·Eduardo CorredorTomás Naranjo
Nov 28, 2007·Nature Reviews. Genetics·Adi Barzel, Martin Kupiec
Dec 7, 2007·Genes & Development·Robert Schneider, Rudolf Grosschedl
Sep 16, 2008·Genetics·Uwe RauwolfStephan Greiner
Oct 29, 2008·Genome Génome / Conseil National De Recherches Canada·Hieronim GolczykStephan Greiner
Mar 12, 2009·Nature Reviews. Molecular Cell Biology·Tom Misteli, Evi Soutoglou
Mar 27, 2009·Current Opinion in Genetics & Development·Elena Fedorova, Daniele Zink
Sep 11, 2009·Nature·Anita Göndör, Rolf Ohlsson
Sep 25, 2010·Cellular & Molecular Biology Letters·Hieronim GolczykMarek Szklarczyk
Feb 15, 2013·The Journal of Investigative Dermatology·Michal R GdulaVladimir A Botchkarev
Apr 3, 2014·Current Opinion in Cell Biology·Jan Padeken, Patrick Heun
Nov 29, 2014·Frontiers in Genetics·Veit SchubertIngo Schubert
Apr 18, 2015·Genome Research·Patrick J WijchersWouter de Laat
Oct 1, 2015·Annual Review of Genetics·Olivier Da Ines, Charles I White
Feb 20, 2016·Chromosoma·Célia BarouxUeli Grossniklaus
Apr 12, 2016·Current Opinion in Genetics & Development·Eric F JoyceC-Ting Wu
Aug 2, 2017·Neurochemistry International·Yuki Fujita, Toshihide Yamashita

❮ Previous
Next ❯

Software Mentioned

NIS Elements

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved