Pericytes augment the capillary barrier in in vitro cocultures

The Journal of Surgical Research
C J DenteJames G Tyburski

Abstract

Most in vitro studies of capillary permeability focus on endothelial cell (MVEC) monolayers and ignore the second cell that forms the capillary wall: the microvascular pericyte (PC). We describe a model to study the permeability characteristics of MVEC, PC, and MVEC:PC cocultures. Semipermeable culture inserts were coated with collagen and then plated with early passage bovine pulmonary MVEC. On Day 3, bovine pulmonary PC were added at concentrations to approximate MVEC:PC ratios of 1:1, 5:1, and 10:1. Electrical resistance was measured on subsequent days and fluorescently labeled (FITC) albumin was used in a permeability assay to calculate an albumin clearance for each culture. The results for electrical resistance measurements and albumin assays showed a similar pattern. Resistance for endothelial cell monolayers was significantly higher and albumin permeability was significantly lower than that of controls. Addition of pericytes at a 10:1 and 5:1 ratios increased the permeability barrier compared to endothelial cells alone, although these cultures were not significantly different from one another. Cocultures at a 1:1 ratio had the best barrier, significantly better than all other cultures. Endothelial cell monolayers are an ...Continue Reading

References

Dec 1, 1992·Cell and Tissue Research·V NehlsD Drenckhahn
Jan 1, 1986·Tissue & Cell·D E Sims
Sep 1, 1987·The Journal of Cell Biology·A Orlidge, P A D'Amore
Jan 1, 1996·Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift für Augenheilkunde·D R Anderson, E B Davis

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Citations

Apr 3, 2012·Cancer Microenvironment : Official Journal of the International Cancer Microenvironment Society·Keith D BarlowLinda J Metheny-Barlow
Sep 21, 2013·Cellular and Molecular Life Sciences : CMLS·Yao Yao, Stella E Tsirka
Jun 7, 2013·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Felix DyrnaIngo Bechmann
May 19, 2007·The Journal of Surgical Research·David A EdelmanChristopher P Steffes
May 22, 2007·American Journal of Surgery·David A EdelmanChristopher P Steffes
Jun 18, 2009·Glia·Martin Krueger, Ingo Bechmann
Aug 26, 2006·The Journal of Surgical Research·David A EdelmanChristopher Steffes
Apr 25, 2006·The Journal of Surgical Research·David A EdelmanChristopher Steffes
Feb 24, 2006·American Journal of Surgery·Lydia DonoghueRobert F Wilson
Jul 23, 2013·Current Opinion in Neurobiology·Julie A SiegenthalerRichard Daneman
Aug 16, 2011·Developmental Cell·Annika ArmulikChrister Betsholtz
Dec 6, 2014·Archives of Medical Research·Maria Alexandra BritoDora Brites
Sep 6, 2005·The Journal of Surgical Research·Sid KerkarChristopher P Steffes
Jul 15, 2005·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Jeong Ai KimMark J Fisher
Jun 19, 2014·Stroke; a Journal of Cerebral Circulation·Yarong HeYu Cao
Jan 12, 2007·The Journal of Trauma·David A EdelmanChristopher P Steffes
Nov 1, 2018·American Journal of Physiology. Cell Physiology·Changjun YangEduardo Candelario-Jalil
Jan 13, 2019·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Lefeng WangSanjay Mehta
Nov 1, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Stephan NeesGerd Juchem
Mar 20, 2016·American Journal of Physiology. Heart and Circulatory Physiology·Valerie ArpinoSean E Gill
Feb 16, 2020·Journal of Neuroscience Research·Clémence Disdier, Barbara S Stonestreet
Jun 17, 2020·Frontiers in Physiology·Pingnian HeFeng Gao
Jul 19, 2013·Biology Open·Julie A SiegenthalerSamuel J Pleasure

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