PMID: 7018252Jun 11, 1981Paper

Perifused pituitary cultures: a model for LHRH regulation of LH secretion

The American Journal of Physiology
J S LoughlinW F Crowley

Abstract

In this paper, a dispersed anterior pituitary cell system that is continuously perifused and suitable for the study of luteinizing hormone-releasing hormone (LHRH)-anterior pituitary physiology is described. Adult male Sprague-Dawley rats were decapitated and the anterior pituitaries removed, dispersed using collagenase, mixed with Biogel P2, and packed into 0.9-cm columns using sterile technique. All experiments were conducted using Krebs-Ringer-bicarbonate-glucose buffer, and a stable base line was achieved. A constant dose of LHRH (50 ng) administered several times at 1-h intervals stimulated the same quantal release of LH. LH secretion is linear in response to LHRH over the range of 1-50 nM and to rat hypothalamic extracts over a range of 1/32 to 1/2 hypothalamic equivalents, regardless of whether the extracts are administered in ascending or descending order. These results indicate that perifusion of dispersed pituitary cells provides a useful model for the study of LHRH physiology, combining the advantages of the long-term viability of pituitary cells in culture with the dynamic nature afforded by perifusion.

References

Mar 1, 1977·Journal of Reproduction and Fertility·M J KilpatrickW P Collins
Jun 1, 1976·Molecular and Cellular Endocrinology·J de KoningG P van Rees
Nov 1, 1976·Endocrinology·J E Martin, D C Klein
Jan 11, 1973·The American Journal of Physiology·R BlackwellR Guillemin
Oct 1, 1973·The Journal of Clinical Investigation·R J Santen, C W Bardin
Jul 1, 1972·The Journal of Clinical Endocrinology and Metabolism·R BoyarE Weitzman
Dec 1, 1971·The Journal of Clinical Endocrinology and Metabolism·A R Midgley, R B Jaffe
Apr 1, 1970·Proceedings of the Society for Experimental Biology and Medicine·G B Serra, A R Midgley

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Citations

Apr 28, 2011·American Journal of Physiology. Endocrinology and Metabolism·E ZhaoVance L Trudeau

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