PMID: 4291493Feb 1, 1967Paper

Periodate oxidation of sperm-whale myoglobin and the role of the methionine residues in the antigen-antibody reaction

The Biochemical Journal
M Z Atassi

Abstract

1. Oxidation of sperm-whale metmyoglobin and its apoprotein with periodate has been investigated under various conditions of pH and temperature to find those under which the reagent acted with specificity. 2. At pH6.8 and 22 degrees consumption of periodate ceased in 3(1/2)hr. at 43 moles of periodate/mole of myoglobin. The two methionine residues, the two tryptophan residues, the three tyrosine residues and two histidine residues were oxidized; serine increased in the hydrolysates from 6 to 9 residues/mol. 3. At pH5.0 and 22 degrees , consumption levelled off in 4(1/2)hr. at 26 moles of periodate/mole of myoglobin and resulted in the modification of the two methionine residues, the two tryptophan residues, the three tyrosine residues and two histidine residues; serine increased from 6 to 7 residues/mol. and, also, ferrihaem suffered considerable oxidation. 4. Oxidation at pH5.0 and 0 degrees resulted at completion (4hr.) in the consumption of 22 moles of periodate/mole of myoglobin and in the modification of the methionine, tyrosine and tryptophan residues. Spectral studies indicated oxidation of the haem group. This derivative reacted very poorly with rabbit antisera to MbX (the major component no. 10 obtained by CM-cellulose...Continue Reading

Citations

Jan 1, 1979·CRC Critical Reviews in Biochemistry·M Z Atassi
Feb 26, 1982·Biochemical and Biophysical Research Communications·B D Howard
May 1, 1970·Archives of Biochemistry and Biophysics·P Azari, J L Phillips
Jul 24, 1972·European Journal of Biochemistry·H RochatS Lissitzky
Feb 1, 1975·The Australian Journal of Experimental Biology and Medical Science·K J Lafferty, A J Cunningham
Jan 1, 1978·Immunochemistry·A L Kazim, M Z Atassi
Nov 8, 1976·Biochimica Et Biophysica Acta·F M van WezelG H de Haas
Aug 30, 1973·Biochimica Et Biophysica Acta·J M Gleisner, I E Liener
Jan 1, 1969·Immunochemistry·M Z Atassi, D J Skalski
Jan 1, 1968·Immunochemistry·J E May, R K Brown

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