Periodic expression of cell-cycle regulators: A laboratory experiment proposal for students in molecular and cell biology

Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology
Inma Quilis, J Carlos Igual

Abstract

This article describes a laboratory exercise designed for undergraduate students in the subject of "Regulation of cell proliferation" which allows the students to carry out a research experiment in an important field such as cell cycle control, and to be introduced to a widely used technique in molecular biology laboratories such as the western blot. The cell cycle is regulated by the succession of cyclin-CDK kinase activities. Activation and inactivation of different cyclin-CDK complexes depend on the control of their positive and negative regulators, cyclins and CDK inhibitors (CKIs), respectively. In this experiment, fluctuations in the level of mitotic cyclin Clb2 and CDK inhibitor Sic1 throughout the cell cycle of Saccharomyces cerevisiae are analyzed, particularly in the context of the control of mitotic exit and Start, two of the most important cell cycle transitions. In order to do this, a cdc15 mutant strain is used to block cells in telophase and, upon release from this blocking, the variation in the levels of Clb2 and Sic1 proteins are analyzed by western blot. Progress along the cell cycle is also evaluated by microscopic analysis of cell morphology and nuclear staining. This practical illustrates the experimental b...Continue Reading

References

Dec 5, 1998·Microbiology and Molecular Biology Reviews : MMBR·M D Mendenhall, A E Hodge
Feb 27, 2001·FEBS Letters·L M DeSalle, M Pagano
Jan 16, 2003·Current Biology : CB·Linda L Breeden
Dec 1, 2004·Annual Review of Genetics·Frank Stegmeier, Angelika Amon
May 11, 2005·Cell·William J Bosl, Rong Li
Apr 25, 2006·Nature Reviews. Cancer·Keiichi I Nakayama, Keiko Nakayama
Aug 10, 2006·Nature Reviews. Molecular Cell Biology·Jan-Michael Peters
Jul 4, 2007·Journal of Cell Science·M Angeles JuanesJ Carlos Igual
Feb 12, 2011·Advances in Genetics·Christopher J McInerny
Nov 24, 2011·PLoS Biology·Matthew J KloserTadashi Fukami
Jul 24, 2013·Nature Reviews. Molecular Cell Biology·Cosetta BertoliRobertus A M de Bruin
Aug 7, 2013·Current Opinion in Cell Biology·Amy Johnson, Jan M Skotheim

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.

Cell Cycle Pathways

Cell cycle is a complex process regulated by several signal transduction pathways and enzymes. Here is the latest research on regulation of cell cycle and cell cycle pathways.

Related Papers

Seminars in Cancer Biology
J Pines
The Korean Journal of Internal Medicine
W H KimJ K Ko
Results and Problems in Cell Differentiation
T J SoosA Koff
Sheng li ke xue jin zhan [Progress in physiology]
H Jiang, Y C Wang
© 2022 Meta ULC. All rights reserved