Periodic hyperpolarizing responses in hamster and mouse eggs fertilized with mouse sperm

The Journal of Physiology
Y IgusaN Yamashita

Abstract

1. The zona-free hamster egg allows multiple entries of heterologous as well as homologous sperm. The hamster egg inseminated with mouse sperm (M x H egg) showed recurring, transient hyperpolarizing responses (h.r.s) with the peak of -70 to -80 mV. They were superimposed on a hyperpolarizing shift of the resting potential (h.s.) which gradually reached -60 mV in 50 min after insemination.2. Unlike the hamster sperm, the cessation of flagellar motion of the first mouse sperm (;1-stop') failed to induce the first h.r. but produced only a small hyperpolarizing ;step' of 3-7 mV. Similar steps occurred for each of additional sperm with a one-to-one correspondence, 4-50 sec ahead of the cessation of sperm motion.3. In M x H eggs, the h.r. first appeared about 15 min after the ;1-stop'. The intervals of the h.r.s thereafter were in the range between 2-10 min, in contrast to 30-45 sec in hamster eggs inseminated with hamster sperm (H x H eggs).4. The h.r.s in M x H eggs were abolished by intracellular injection of EGTA, suggesting that they were caused by periodic increase in the intracellular Ca(2+) concentration ([Ca(2+)](i)) as in H x H eggs.5. The gradual h.s. in M x H eggs was considered to be due mainly to an increase in Ca-indep...Continue Reading

Citations

Feb 20, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Chunmin Wang, Zoltan Machaty
Feb 20, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Allison J GardnerJanice P Evans
Apr 29, 2014·Biochemical and Biophysical Research Communications·Junaid KashirKarl Swann
Jul 1, 1985·Cell Differentiation·M SimonneauH Jakob
Jan 5, 2016·Cell Calcium·Hoi Chang LeeIngrid Carvacho
Oct 11, 2015·Cell and Tissue Research·Zoltan Machaty
Feb 1, 1991·Cell Calcium·S Miyazaki
Dec 10, 2013·Cell Reports·Ingrid CarvachoDavid E Clapham
Jun 10, 2016·Human Reproduction Update·Elisabetta Tosti, Yves Ménézo
Mar 28, 2006·Current Topics in Developmental Biology·Julian L Wong, Gary M Wessel
May 1, 1997·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·K Swann, F A Lai
Jun 1, 1994·Molecular Reproduction and Development·L GianaroliB Dale
Oct 1, 1985·The Journal of Experimental Zoology·D KlineR P Tucker
Jan 26, 2010·Marine Drugs·Francesco Silvestre, Elisabetta Tosti
Feb 1, 1994·Zygote : the Biology of Gametes and Early Embryos·C R House
May 1, 1988·Pflügers Archiv : European journal of physiology·A PeresG Mostacciuolo
Nov 1, 1986·Pflügers Archiv : European journal of physiology·A Peres
Feb 1, 1993·The Journal of Experimental Zoology·L F Rickords, K L White
Feb 1, 1993·Molecular Reproduction and Development·P CollasF L Barnes
Nov 10, 2018·Molecular Reproduction and Development·John ParringtonRafael A Fissore
May 11, 2006·Reproductive Biology and Endocrinology : RB&E·Elisabetta Tosti
Oct 1, 1985·Pflügers Archiv : European journal of physiology·H ChangK Kurokawa
Dec 24, 2005·Physiological Reviews·Michael Whitaker
Nov 1, 1994·Zygote : the Biology of Gametes and Early Embryos·B Dale
Feb 1, 1993·Zygote : the Biology of Gametes and Early Embryos·J M Murnane, L J DeFelice
Dec 4, 2015·Physiological Reviews·Karl Swann, F Anthony Lai
Aug 21, 2019·Cold Spring Harbor Perspectives in Biology·Takuya WakaiRafael A Fissore
Jul 17, 2020·Open Biology·Paula SteinCarmen J Williams
Jul 13, 2018·Frontiers in Cell and Developmental Biology·Ingrid CarvachoKhaled Machaca
Apr 30, 1998·Physiological Reviews·K Takahashi, Y Okamura
Feb 16, 2006·Reproduction, Fertility, and Development·Allison J Gardner, Janice P Evans
Mar 29, 2020·Molecular Reproduction and Development·Janice P Evans
Oct 21, 2015·Journal of Cell Science·Miranda L BernhardtCarmen J Williams
Apr 1, 1983·Developmental Biology·L A JaffeD P Wolf
Jan 30, 1987·Science·P M Wassarman

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.