Periodic Pattern of Genetic and Fitness Diversity during Evolution of an Artificial Cell-Like System

Molecular Biology and Evolution
Norikazu IchihashiTetsuya Yomo

Abstract

Genetic and phenotypic diversity are the basis of evolution. Despite their importance, however, little is known about how they change over the course of evolution. In this study, we analyzed the dynamics of the adaptive evolution of a simple evolvable artificial cell-like system using single-molecule real-time sequencing technology that reads an entire single artificial genome. We found that the genomic RNA population increases in fitness intermittently, correlating with a periodic pattern of genetic and fitness diversity produced by repeated diversification and domination. In the diversification phase, a genomic RNA population spreads within a genetic space by accumulating mutations until mutants with higher fitness are generated, resulting in an increase in fitness diversity. In the domination phase, the mutants with higher fitness dominate, decreasing both the fitness and genetic diversity. This study reveals the dynamic nature of genetic and fitness diversity during adaptive evolution and demonstrates the utility of a simplified artificial cell-like system to study evolution at an unprecedented resolution.

References

Mar 22, 1994·Proceedings. Biological Sciences·P SchusterI L Hofacker
Nov 1, 1994·Biological Reviews of the Cambridge Philosophical Society·A W Edwards
Aug 2, 2001·Nature Biotechnology·Y ShimizuT Ueda
Mar 15, 2005·FEBS Letters·Andreas Wagner
Sep 13, 2008·Chembiochem : a European Journal of Chemical Biology·Hiroshi KitaTetsuya Yomo
Jun 10, 2009·Bioinformatics·Heng LiUNKNOWN 1000 Genome Project Data Processing Subgroup
Sep 25, 2009·Cold Spring Harbor Symposia on Quantitative Biology·J E Barrick, R E Lenski
Jan 19, 2010·Bioinformatics·Heng Li, Richard Durbin
Oct 16, 2010·Science·Jason N Pitt, Adrian R Ferré-D'Amaré
May 7, 2011·Genetics·Gregory I LangMichael M Desai
Aug 28, 2013·Proceedings of the National Academy of Sciences of the United States of America·José I JiménezIrene A Chen
Oct 4, 2013·Nature Communications·Norikazu IchihashiTetsuya Yomo

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Citations

Apr 2, 2016·Proceedings of the National Academy of Sciences of the United States of America·Yohsuke BanshoTetsuya Yomo

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