Periodic surface array in Caulobacter crescentus: fine structure and chemical analysis.

Journal of Bacteriology
J SmitN Agabian

Abstract

A periodic array structure on the cell surface of Caulobacter crescentus CB15 was revealed by electron microscopy of the cell envelope, using negative staining, thin-sectioning, and freeze-etching. This structural layer has been isolated from liquid cultures, in which large pieces of the two-dimensional array are shed by cells grown to high density. Often areas of intact array corresponding to the entire cell surface could be found. The hexagonally arranged structure was highly ordered and had an unusual degree of complexity, as determined by optical diffraction and computer processing of micrographs of negatively stained, isolated surface array. Filtered, reconstructed images were obtained from both normal and low-electron-dose micrographs demonstrating resolutions of 2.9 and 25 nm, respectively. Comparison by optical diffraction and image filtering of micrographs recorded by using either normal or minimal beam exposure techniques suggested that the lower-resolution features of the image are very stable to electron exposure. Gel electrophoresis indicated that isolated array preparations contain a number of polypeptides. It appears likely that more than one of these proteins are structural components of the array, in contrast t...Continue Reading

References

Apr 1, 1976·Canadian Journal of Microbiology·T J Beveridge, R G E Murray
Apr 1, 1976·Journal of Ultrastructure Research·T J Beveridge, R G Murray
Jan 1, 1976·Annual Review of Microbiology·L Shapiro
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·U AebiM K Showe
Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·W Baumeister, O Kübler
Mar 1, 1979·Journal of Ultrastructure Research·R M GlaeserD Grano
Sep 1, 1978·Journal of Bacteriology·J S Poindexter
Oct 1, 1976·Journal of Bacteriology·C Lagenaur, N Agabian
Dec 20, 1976·Biochimica Et Biophysica Acta·K J ThorneM F Heath
Feb 1, 1975·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L L Muller, T J Jacks
Dec 1, 1975·Journal of Bacteriology·T J Beveridge, R G Murray
Nov 1, 1975·Canadian Journal of Microbiology·H F RidgwayR A Lewin
Aug 28, 1970·Journal of Molecular Biology·R C Williams, H W Fisher
Apr 1, 1971·Virology·N Agabian-Keshishian, L Shapiro
Jan 1, 1973·Canadian Journal of Microbiology·F L Buckmire, R G Murray
Jan 1, 1969·Annual Review of Microbiology·A M Glauert, M J Thornley
May 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·L ShapiroO M Rosen
Oct 1, 1973·The Review of Scientific Instruments·K A Taylor, R M Glaeser
Jan 1, 1972·Archiv für Mikrobiologie·F Hollaus, U Sleytr
Oct 1, 1970·Journal of Ultrastructure Research·S W Watson, C C Remsen
Jan 1, 1967·Archiv für Mikrobiologie·J S PoindexterP A Armstrong
Jun 1, 1967·Journal of Bacteriology·M V Nermut, R G Murray
Feb 15, 1980·Journal of Molecular Biology·M StewartR G Murray
Apr 1, 1980·Journal of Bacteriology·M Stewart, T J Beveridge
Feb 1, 1957·Journal of Cellular Physiology·G COHEN-BAZIRER Y STANIER
Sep 1, 1964·Bacteriological Reviews·J S POINDEXTER
Dec 1, 1964·The Journal of Cell Biology·J L STOVEPOINDEXTER, G COHEN-BAZIRE

❮ Previous
Next ❯

Citations

Sep 8, 2016·Acta Crystallographica. Section F, Structural Biology Communications·Michael D JonesJohn Smit
Sep 14, 2010·Journal of Bacteriology·Fernando AmatMark Horowitz
Dec 22, 2009·Journal of Bacteriology·Constantin N TakacsChristine Jacobs-Wagner
Jun 1, 1997·FEMS Microbiology Reviews·T J BeveridgeS F Koval
Sep 1, 2018·Canadian Journal of Microbiology·Cezar M KhursigaraRobert J Harris
Aug 1, 1988·Applied and Environmental Microbiology·R I Merker, J Smit
Apr 1, 1992·Journal of Bacteriology·D A StahlJ Smit
Jan 1, 1992·Electron Microscopy Reviews·L L Graham
Oct 1, 1988·Journal of Bacteriology·J A FisherN Agabian
Mar 1, 1991·Applied and Environmental Microbiology·J D MacRae, J Smit
Jan 1, 1991·Journal of Bacteriology·A Gilchrist, J Smit
Jan 1, 1982·Developmental Biology·J Smit, N Agabian

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.