Periodicity in recA protein-DNA complexes

FEBS Letters
Alexander A VolodinT N Bocharova

Abstract

The reaction of guanine residues with dimethylsulfate was studied for complexes of recA protein with fluorescent dye tagged double stranded oligonucleotides. The patterns of dimethylsulfate modification obtained demonstrate a similarity of DNA states in the complexes with recA protein formed as a result of recA promoted strand exchange and renaturation reactions. The guanine modification efficiency varies periodically as a function of the base position along the oligonucleotide axis, with a period of 3 nucleotides. This effect suggests that the arrangement of recA monomers along the oligonucleotide is strictly ordered, and the dimethylsulfate reactivity of a guanine residue depends on the site of its binding in a recA monomer.

References

Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A M Maxam, W Gilbert
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Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·U Siebenlist, W Gilbert

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Citations

Nov 24, 1999·Cancer Genetics and Cytogenetics·S E IngrahamA G Menon
Apr 8, 2000·Trends in Biochemical Sciences·S C Kowalczykowski
Apr 8, 2000·Trends in Biochemical Sciences·E Egelman
Jan 1, 2000·Journal of Biomolecular Structure & Dynamics·F GuoG D Van Duyne
Jan 24, 2015·Colloids and Surfaces. B, Biointerfaces·Shusuke OuraKazuo Umemura
Jul 2, 2003·FEBS Letters·Alexander A VolodinR Daniel Camerini-Otero
Nov 9, 2001·The Journal of Biological Chemistry·Alexander A Volodin, R Daniel Camerini-Otero

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