Perioperative gene expression analysis for prediction of postoperative sepsis

Clinical Chemistry
Carl HinrichsDidier Keh

Abstract

Postoperative sepsis is one of the main causes of death after major abdominal surgery; however, the immunologic factors contributing to the development of sepsis are not completely understood. In this study, we evaluated gene expression in patients who developed postoperative sepsis and in patients with an uncomplicated postoperative course. We enrolled 220 patients in a retrospective matched-pair, case-control pilot study to investigate the perioperative expression of 23 inflammation-related genes regarding their properties for predicting postoperative sepsis. Twenty patients exhibiting symptoms of sepsis in the first 14 days after surgery (case group) were matched with 20 control patients with an uncomplicated postoperative course. Matching criteria were sex, age, main diagnosis, type of surgery, and concomitant diseases. Blood samples were drawn before surgery and on the first and second postoperative days. Relative gene expression was analyzed with real-time reverse-transcription PCR. Significant differences (P < 0.005) in gene expression between the 2 groups were observed for IL1B (interleukin 1, beta), TNF [tumor necrosis factor (TNF superfamily, member 2)], CD3D [CD3d molecule, delta (CD3-TCR complex)], and PRF1 [perfori...Continue Reading

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Citations

Mar 23, 2011·Critical Care : the Official Journal of the Critical Care Forum·Caroline GuignantFabienne Venet
Oct 27, 2011·Critical Care : the Official Journal of the Critical Care Forum·Fanny Turrel-DavinGuillaume Monneret
Dec 11, 2013·Critical Care : the Official Journal of the Critical Care Forum·Marie-Angélique CazalisFabienne Venet
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Jun 17, 2011·Current Opinion in Critical Care·Elchanan FriedCharles Sprung

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