Periosteum-derived mesenchymal progenitor cells in engineered implants promote fracture healing in a critical-size defect rat model

Journal of Tissue Engineering and Regenerative Medicine
Ana B González-GilFroilán Granero-Moltó

Abstract

An attractive alternative to bone autografts is the use of autologous mesenchymal progenitor cells (MSCs) in combination with biomaterials. We compared the therapeutic potential of different sources of mesenchymal stem cells in combination with biomaterials in a bone nonunion model. A critical-size defect was created in Sprague-Dawley rats. Animals were divided into six groups, depending on the treatment to be applied: bone defect was left empty (CTL); treated with live bone allograft (LBA); hrBMP-2 in collagen scaffold (CSBMP2 ); acellular polycaprolactone scaffold (PCL group); PCL scaffold containing periosteum-derived MSCs (PCLPMSCs ) and PCL containing bone marrow-derived MSCs (PCLBMSCs ). To facilitate cell tracking, both MSCs and bone graft were isolated from green fluorescent protein (GFP)-transgenic rats. CTL group did not show any signs of healing during the radiological follow-up (n = 6). In the LBA group, all the animals showed bone bridging (n = 6) whereas in the CSBMP2 group, four out of six animals demonstrated healing. In PCL and PCLPMSCs groups, a reduced number of animals showed radiological healing, whereas no healing was detected in the PCLBMSCs group. Using microcomputed tomography, the bone volume filling t...Continue Reading

References

Aug 26, 1998·Genes & Development·M Whitman
Apr 2, 1999·Science·M F PittengerD R Marshak
Jan 12, 2001·Clinical Orthopaedics and Related Research·F J HornicekH J Mankin
Jan 5, 2002·Differentiation; Research in Biological Diversity·A AsakuraM Rudnicki
Jul 16, 2003·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Dennis RohnerBeat Hammer
Sep 12, 2003·Stem Cells·Ranieri CanceddaRodolfo Quarto
Nov 19, 2003·Advanced Drug Delivery Reviews·M GeigerW Friess
Jul 14, 2004·Yonsei Medical Journal·Hajime OhgushiYoshinori Takakura
Nov 19, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Xinping ZhangRobert E Guldberg
Mar 27, 2007·Injury·Robert BielbyDennis McGonagle
May 15, 2007·Circulation Research·Jeffrey M GimbleBruce A Bunnell
Jul 20, 2007·ANZ Journal of Surgery·Oliver P GautschiRené Zellweger
Mar 20, 2008·The Journal of Bone and Joint Surgery. American Volume·Chao XieXinping Zhang
Oct 14, 2008·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Céline Colnot
May 5, 2009·The Journal of Bone and Joint Surgery. American Volume·Anna V CuomoJay R Lieberman
Jun 23, 2009·Stem Cells·Froilán Granero-MoltóAnna Spagnoli
May 7, 2011·Clinical Orthopaedics and Related Research·Michael D BallMolly M Stevens
Jun 22, 2011·Clinical Orthopaedics and Related Research·Luis Aponte-TinaoD Luis Muscolo
May 4, 2012·The Journal of the American Academy of Orthopaedic Surgeons·Julius A BishopDavid W Lowenberg
Jul 6, 2012·Science Translational Medicine·Johannes C ReichertDietmar W Hutmacher
Jul 11, 2012·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Céline ColnotMelissa L Knothe Tate
Apr 30, 2013·Biomaterials·Cédryck VaquetteDietmar W Hutmacher
Nov 22, 2013·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Chelsea S BahneyRalph S Marcucio
Feb 1, 2014·Methods in Molecular Biology·Elise F MorganLouis C Gerstenfeld
Feb 15, 2014·Advances in Wound Care·M Noelle Knight, Kurt D Hankenson

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Citations

Sep 16, 2020·Journal of Cellular and Molecular Medicine·Han XiaoHongbin Lu
Jun 3, 2021·International Journal of Molecular Sciences·Agnese GugliandoloFrancesca Diomede
Jun 27, 2021·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·José Valdés-FernándezFroilán Granero-Moltó

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