Periostin, a signal transduction intermediate in TGF-β-induced EMT in U-87MG human glioblastoma cells, and its inhibition by anthocyanidins

Oncotarget
Amira OuanoukiBorhane Annabi

Abstract

Periostin is a secreted protein that is highly expressed in glioblastoma cells as compared to normal brain tissue, and is therefore considered as a potential biomarker in therapeutic modalities. Its contribution in the cancer cells invasive phenotype is, however, poorly understood. This work investigates the role of periostin in U-87 MG glioblastoma cell invasion, cell migration and in Transforming Growth Factor β (TGF-β)-induced epithelial-mesenchymal transition (EMT). Periostin gene silencing, using small interfering RNA, decreased TGF-β-induced mesenchymal marker expression of fibronectin and vimentin, partly through reduced Smad2, Akt and Fak phosphorylation as well as U-87 MG cell invasion and migration. The effects of anthocyanidins, the most abundant diet-derived flavonoids, were examined on periostin-mediated downstream signaling pathways. Anthocyanidins were found to decrease periostin expression whether added under pre-, co- or post-treatment conditions along with TGF-β, and altered the Akt and Fak signaling pathways. These effects were similar to Galunisertib (LY2157299), a small molecule inhibitor of the TGF-β receptor I kinase. Taken together, our data demonstrate that periostin acts as a central element in TGF-β-i...Continue Reading

References

Sep 5, 2002·Journal of Agricultural and Food Chemistry·Navindra P Seeram, Muraleedharan G Nair
May 2, 2003·Nature Reviews. Cancer·Peter Friedl, Katarina Wolf
Sep 1, 2005·Journal of Agricultural and Food Chemistry·Sabina PassamontiFulvio Mattivi
Aug 24, 2006·Biochimica Et Biophysica Acta·Hideki Yamaguchi, John Condeelis
Jul 10, 2007·Histology and Histopathology·Y KudoT Takata
Jan 27, 2009·Cancer Metastasis Reviews·Mahmut Yilmaz, Gerhard Christofori
Mar 25, 2009·Cellular and Molecular Life Sciences : CMLS·Kai RuanGaoliang Ouyang
Apr 14, 2009·Cancer Chemotherapy and Pharmacology·Timothy H MarczyloAndreas J Gescher
Jun 3, 2009·The Journal of Clinical Investigation·Hervé AcloqueM Angela Nieto
Oct 15, 2011·Virchows Archiv : an International Journal of Pathology·Laura Morra, Holger Moch
Jun 28, 2012·International Journal of Oncology·Kusumawadee UtispanChanitra Thuwajit
Feb 5, 2013·Brain : a Journal of Neurology·Patrick RothMichael Weller
May 9, 2013·Expert Opinion on Therapeutic Targets·Lior H KatzLopa Mishra
Oct 23, 2013·Cellular and Molecular Life Sciences : CMLS·Simon J ConwayAkira Kudo
Dec 1, 2010·Toxicological Research·Hwajin Son, Aree Moon
Aug 12, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Hae-Yun JungJing Yang
Aug 21, 2014·Neuro-oncology·Andrei M MikheevRobert C Rostomily
Oct 18, 2016·Critical Reviews in Food Science and Nutrition·Hiu Yee KwanZhi-Ling Yu
Apr 29, 2015·Oncotarget·Shailendra Kumar Dhar DwivediResham Bhattacharya
May 30, 2015·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Qingfeng HuGuowei Xia
Jul 8, 2015·Folia Histochemica Et Cytobiologica·Katarzyna Ratajczak-Wielgomas, Piotr Dziegiel
Sep 12, 2015·Journal of Cancer Research and Clinical Oncology·Jie ZhangYu Jiang
Feb 4, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sadia AfrinMaurizio Battino
Mar 16, 2016·Scientific Reports·Chao ZhangXingang Li
Mar 27, 2016·British Journal of Clinical Pharmacology·Chiara CerlettiMaria Benedetta Donati
Jun 17, 2016·Molecular Cancer Therapeutics·Soon Young ParkJohn F de Groot
Oct 21, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Mayra PaolilloSergio Schinelli
Oct 27, 2016·British Journal of Pharmacology·Bo-Wen LinYing-Yu Cui
Nov 10, 2016·Journal of Clinical Medicine·Audrey LamoraFranck Verrecchia
Nov 29, 2016·Oncotarget·Nicola Di DanieleAntonino De Lorenzo
Jan 14, 2017·Molecular Oncology·Kay T Yeung, Jing Yang

❮ Previous
Next ❯

Citations

Sep 11, 2019·American Journal of Respiratory Cell and Molecular Biology·Yasuhiro NanriKenji Izuhara
Feb 6, 2020·International Journal of Molecular Sciences·Aleksandra Ellert-MiklaszewskaBozena Kaminska
Jul 20, 2019·Molecular Medicine Reports·Chao-Yang DuanYan Lin
Aug 20, 2019·Oncology Letters·Fengyu ZhaiYi Zhang

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence microscopy
PCR
protein assay
electrophoresis
confocal microscopy
transfections

Software Mentioned

GraphPad Prism
CFX Manager
ImageJ
QuantiTect
xCELLigence Real - Time Cell Analysis system

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.