Periostin Enhances Migration, Invasion, and Adhesion of Human Endometrial Stromal Cells Through Integrin-Linked Kinase 1/Akt Signaling Pathway

Reproductive Sciences
Xiaoxuan XuPeishu Liu

Abstract

Although our previous study confirmed that periostin (PN) was overexpressed in the eutopic and ectopic endometrial stroma of women with endometriosis by immunohistochemitry, the role of PN in the pathophysiology of endometriosis remains unknown. Thus, we aimed to investigate the effects of PN on endometrial stromal cells (ESCs) migration, invasion, adhesion, and proliferation and to further study the mechanism under this process. Eutopic (EuSCs), ectopic (EcSCs), and normal ESCs (NSCs) were isolated and cultured. We evaluated the above-mentioned biology behaviors and the expression of PN, integrin-linked kinase 1 (ILK1), and phospho-Akt (p-Akt) in NSCs, EuSCs as well as EcSCs before and after receiving PN small-interfering RNA (siRNA). The protein and messenger RNA (mRNA) levels of PN were upregulated in EuSCs (P < .05; P = .2261 in proliferative phase and P = .3385 in secretory phase) and EcSCs (P < .001; P < .001 in proliferative phase and P < .05 in secretory phase) compared with NSCs, although there was no significant difference in PN mRNA between EuSCs and NSCs. In EcSCs, abilities of migration, invasion, and adhesion and the expressions of ILK1 and p-Akt were enhanced; and all of those were downregulated after PN siRNA in...Continue Reading

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Citations

Jun 3, 2016·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Qiaomei ZhengPeishu Liu
Feb 28, 2018·Chinese Medical Journal·Xiao-Xuan XuJing-He Lang
Mar 2, 2018·Reproductive Sciences·Xiaoxuan XuJinghe Lang
Sep 6, 2018·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Qiao-Mei ZhengLi-Hong Chen
Oct 13, 2018·Acta Obstetricia Et Gynecologica Scandinavica·Endah RahmawatiChii-Ruey Tzeng
Sep 19, 2018·International Journal of Molecular Medicine·Wenqu LiXiaoyan Shi

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Methods Mentioned

BETA
hysterectomy
electrophoresis
transfection
PCR

Software Mentioned

Image
Pro Plus
SPSS13
Image J

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