Peripheral blood lymphocytes P-glycoprotein (P-gp, gp-170) expression in allogenic kidney transplant patients

Nephrology
Katarzyna KotrychMarek Droździk

Abstract

P-glycoprotein (gp-170, P-gp) is a transmembrane transporter involved in drug, for example cyclosporine A, efflux from the cells thus limiting their intracellular concentration. Expression of the transporter on the surface of immune competent cells may be associated with poor prognosis in kidney transplant patients. The aim of the present study was to evaluate P-gp expression on the surface of CD4(+), CD8(+), CD19(+) and CD56(+) cells in kidney transplant patients treated with cyclosporine A as a main immunosuppressant, using flow cytometry. It was found that P-gp expression in kidney transplant patients with acute rejection did not differ significantly from transplanted patients without rejection studied in the same period after transplantation, as well as from the healthy controls. Administration of 3-day course of 1,000 mg/24 h methylprednisolone did not affect the expression of P-gp in the studied cells, except for significant elevation in CD56(+) cells, which disappeared at 2 weeks after cessation of steroid administration. Based on the results from the present study it can be concluded that P-gp expression is not a prognostic factor of acute kidney graft rejection.

References

May 3, 2008·Biological & Pharmaceutical Bulletin·Kayo HenmiToshihiko Hirano
Jul 5, 2016·Biological & Pharmaceutical Bulletin·Hideyuki KushiharaTakaaki Kobayashi

Citations

Mar 1, 1991·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·J W Smith
Mar 18, 1999·Transplant International : Official Journal of the European Society for Organ Transplantation·A MelkG Opelz
Oct 12, 1999·Journal of Clinical Pharmacology·A Lo, G J Burckart
Apr 8, 2000·European Journal of Endocrinology·G M Lambert-Messerlian

Related Concepts

Transplanted Kidney
Flow Cytometry
T-Lymphocyte
CD19 Expressing Cell Count
Peripheral Blood
Lymphocytes as Percentage of Blood Leukocytes (Lab Test)
Protoplasm
CD19 Antigens
Cyclosporine
NCAM1 wt Allele

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