Abstract
Adult stem cells provide a promising alternative for the treatment of decompensated liver cirrhosis. Our previous study showed that peripheral blood monocytes (PBMCs) from decompensated liver cirrhosis could differentiate into hepatocyte-like cells in vitro. We here aimed to investigate the differential potential of the PBMCs under liver environment. PBMCs were isolated from one cirrhotic patient, who was mobilized by the recombinant human granulocyte colony stimulating factor for consecutive 3days, and then PBMCs were transplanted into nude mice by tail vein after labeled with PKH26-GL. The location of the transplanted PBMCs was identified by PKH26-GL staining and PRINS for human SRY gene. The expressions of human hepatocyte-markers were detected by immunohistochemistry, RT-PCR, and Western blot analysis. Our results demonstrated that PBMCs from decompensated liver cirrhosis could migrate into the liver of nude mice with human hepatocyte-markers expression, indicating that autologous PBMCs transplantation might be one alternative therapy for the decompensated liver cirrhosis.
References
Oct 1, 1989·Chromosoma·J E KochL Bolund
Jan 1, 1995·Cytogenetics and Cell Genetics·J KochL Bolund
Mar 21, 1998·Science·G FerrariF Mavilio
Jun 28, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·N D TheiseD S Krause
Dec 2, 2000·Science·T R BrazeltonH M Blau
Mar 8, 2002·The New England Journal of Medicine·Martin KörblingZeev Estrov
Feb 1, 2003·Blood·Xiuli WangJan A Nolta
Feb 28, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yong ZhaoEliezer Huberman
Jun 5, 2003·Transplantation·Markus DitschkowskiAhmet H Elmaagacli
Feb 21, 2004·Biochemical and Biophysical Research Communications·Yanglin PanDaiming Fan
Mar 26, 2005·Stem Cells·Jan Schulte am EschStefan B Hosch
May 5, 2005·Blood·Laisvyde StatkuteRichard K Burt
May 10, 2005·Transplantation·Maren RuhnkeFred Fandrich
Dec 24, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Snorri S Thorgeirsson, Joe W Grisham
Mar 25, 2006·Stem Cells·Myrtle Y GordonNagy A Habib
Jun 17, 2006·Stem Cells·Shuji TeraiIsao Sakaida
Sep 23, 2006·The New England Journal of Medicine·Volker SchächingerUNKNOWN REPAIR-AMI Investigators
Oct 19, 2006·Experimental Hematology·Evangelia YannakiAthanasios Fassas
May 15, 2007·Lancet·Jie Liu, Daiming Fan
Aug 29, 2007·American Journal of Hematology·Li YanDaiming Fan
Citations
Aug 24, 2012·Stem Cells and Development·Peng LiXiaodan Jiang
Feb 2, 2012·World Journal of Gastroenterology : WJG·Wen-Ting BinXiao-Lin Shi
Jun 27, 2014·Expert Review of Clinical Immunology·Lina CuiDaiming Fan
Oct 15, 2020·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Bing ZhuShaojie Xin
Oct 30, 2021·British Journal of Pharmacology·Chuanrui MaShu Yang