Peripheral blood monocytes show increased osteoclast differentiation potential compared to bone marrow monocytes

Heliyon
Elina KylmäojaJuha Tuukkanen

Abstract

Bone marrow (BM) and peripheral blood (PB) derived mononuclear cells are precursors of in vitro osteoclast differentiation. However, few studies have compared the phenotypic and functional properties of osteoclasts generated from these sources and the effects of different growth factors on osteoclastogenesis. Both cell types differentiated into functional osteoclasts, but culturing the cells with or without transforming growth factor beta (TGF-β) and dexamethasone revealed differences in their osteoclastogenic capacity. When receptor activator for nuclear factor κB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) were used for differentiation, we did not observe differences in bone resorption activity or expression of osteoclastogenic genes calcitonin receptor (CR) and nuclear factor of activated T-cells (NFATc1) between the osteoclasts formed from the two sources. Addition of TGF-β and dexamethasone led to higher number of nuclei in multinuclear cells and increased expression of tartrate resistant acid phosphatase (TRACP) 5a and 5b, CR and NFATc1 in PB- derived osteoclasts depicting the higher osteoclastogenic potential and responsiveness to TGF-β and dexamethasone in PB monocytes. These results conclude that th...Continue Reading

Citations

Jan 4, 2019·Journal of Translational Medicine·Bodo C Melnik, Gerd Schmitz
Jan 16, 2021·Current Osteoporosis Reports·Sana AnsariSandra Hofmann
Apr 16, 2021·Cancer Metastasis Reviews·Massar Alsamraae, Leah M Cook
Jul 15, 2021·Pathology Oncology Research : POR·Kemal Behzatoglu

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Methods Mentioned

BETA
ELISA
Scanning Electron Microscopy
reverse transcription PCR
PCR

Software Mentioned

SPSS
QCapture
OriginPro
ImageJ

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