Peripheral detection of S100beta during cardiothoracic surgery: what are we really measuring?

The Annals of Thoracic Surgery
Vincent FazioDamir Janigro

Abstract

S100beta has been used in cardiac surgery to identify patients with postoperative neurologic complications. However, extracranial proteins may falsely elevate measurements of serum S100beta;. Objectives of this study were (1) to quantify S100 beta levels in serum and pericardial cavity during coronary artery bypass grafting (CABG), and (2) to identify proteins recognized by standard immunodetection as S100beta. Systemic and pericardial cavity blood from 5 patients undergoing CABG were sampled before, during, and after cardiopulmonary bypass (CPB). A commercially available enzyme-linked immunosorbent assay (ELISA) kit was used to quantify S100beta. Two-dimensional gel electrophoresis, Western blot, and mass spectroscopy were also performed to identify S100a and other proteins. Mean S100beta levels measured by ELISA, systemic and pericardial cavity blood were (in ng x mL(-1)) 1.0 +/- 0.46 and 111 +/- 71 before CPB, 0.6 +/- 0.11 and 113 +/- 54 during CPB, and 1.7 +/- 0.64 and 101 +/- 42 after CPB, respectively. However, gel electrophoresis and Western blot analysis revealed proteins other than S100beta to be present in the pericardial cavity giving a falsely elevated serum S100a levels measured by immunoassay. Mass spectroscopy of...Continue Reading

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Citations

Apr 9, 2005·Canadian Journal of Anaesthesia = Journal Canadien D'anesthésie·Hilary P Grocott
Feb 27, 2007·Epilepsia·Nicola MarchiDamir Janigro
Sep 23, 2008·BMC Clinical Pathology·Sharon EinavYigal Shoshan
Sep 17, 2010·PloS One·Nancy PhamDamir Janigro
Mar 14, 2013·PloS One·Nicola MarchiDamir Janigro
Feb 18, 2010·Biomarkers in Medicine·Matthew B Maas, Karen L Furie
Jul 6, 2014·PloS One·Erin BargerstockDamir Janigro
Nov 7, 2013·Sports Medicine·Serene X T Koh, Jason K W Lee
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Mar 4, 2008·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·N KhaladjM Pichlmaier
Jun 16, 2012·The Annals of Thoracic Surgery·Björn ReinsfeltAnne Westerlind
Aug 16, 2005·Expert Review of Proteomics·Kevin K W WangRonald L Hayes
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Sep 28, 2021·Canadian Journal of Anaesthesia = Journal Canadien D'anesthésie·Derek J RobertsKerry B Goralski

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