Peripheral Inflammatory Hyperalgesia Depends on P2X7 Receptors in Satellite Glial Cells

Frontiers in Physiology
Amanda Ferreira NevesCarlos Amílcar Parada

Abstract

Peripheral inflammatory hyperalgesia depends on the sensitization of primary nociceptive neurons. Inflammation drives molecular alterations not only locally but also in the dorsal root ganglion (DRG) where interleukin-1 beta (IL-1β) and purinoceptors are upregulated. Activation of the P2X7 purinoceptors by ATP is essential for IL-1β maturation and release. At the DRG, P2X7R are expressed by satellite glial cells (SGCs) surrounding sensory neurons soma. Although SGCs have no projections outside the sensory ganglia these cells affect pain signaling through intercellular communication. Therefore, here we investigated whether activation of P2X7R by ATP and the subsequent release of IL-1β in DRG participate in peripheral inflammatory hyperalgesia. Immunofluorescent images confirmed the expression of P2X7R and IL-1β in SGCs of the DRG. The function of P2X7R was then verified using a selective antagonist, A-740003, or antisense for P2X7R administered in the L5-DRG. Inflammation was induced by CFA, carrageenan, IL-1β, or PGE2 administered in rat's hind paw. Blockage of P2X7R at the DRG reduced the mechanical hyperalgesia induced by CFA, and prevented the mechanical hyperalgesia induced by carrageenan or IL-1β, but not PGE2. It was also...Continue Reading

References

Jun 28, 2000·Journal of the Autonomic Nervous System·Y DingJ N Wood
Jul 25, 2000·British Journal of Pharmacology·J M CunhaS H Ferreira
Sep 25, 2002·Physiological Reviews·R Alan North
Dec 17, 2002·Arthritis and Rheumatism·Giacomo Dell'AntonioMaria Elena Ferrero
Sep 23, 2003·Pain·Elizabeth K JosephJon D Levine
Apr 3, 2004·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·G G VivancosS H Ferreira
May 26, 2005·Brain Research. Brain Research Reviews·Menachem Hanani
Jul 12, 2005·Brain Research·Xu-Feng ZhangChar-Chang Shieh
Feb 21, 2006·Trends in Pharmacological Sciences·Geoffrey Burnstock
Mar 21, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Davide FerrariFrancesco Di Virgilio
May 30, 2006·Pharmacology & Therapeutics·Waldiceu A VerriSérgio H Ferreira
Sep 16, 2006·Journal of Neuroscience Methods·L F FerrariS H Ferreira
Feb 8, 2007·Journal of Cell Science·David Brough, Nancy J Rothwell
May 5, 2007·Neuroscience·S McGaraughtyM F Jarvis
May 26, 2007·Proceedings of the National Academy of Sciences of the United States of America·X ZhangL-Y M Huang
Jun 1, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Wei GuoKe Ren
Jun 24, 2008·Purinergic Signalling·William A CarrollMichael F Jarvis
Aug 30, 2008·The Journal of Experimental Medicine·Jonathan MaelfaitRudi Beyaert
Dec 26, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Alexander M BinshtokTarek A Samad
Jan 27, 2009·Brain Research Reviews·Ke Ren, Richard Torres
Apr 7, 2009·European Journal of Pain : EJP·Tian-Ying HuangMenachem Hanani
Jun 30, 2009·British Journal of Pharmacology·Diana L Donnelly-RobertsMichael F Jarvis
Nov 17, 2009·Trends in Neurosciences·Michael F Jarvis
Jan 15, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Anna K ClarkMarzia Malcangio
May 11, 2010·Brain Research Reviews·Claudia Verderio, Michela Matteoli
Jul 14, 2010·European Journal of Pharmacology·Juliana Maia TeixeiraCláudia Herrera Tambeli
Mar 8, 2011·Seminars in Cell & Developmental Biology·Arthur M Butt
Apr 23, 2011·Expert Opinion on Investigational Drugs·Nishkantha ArulkumaranFrederick Wk Tam

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Methods Mentioned

BETA
ELISA
PCR
confocal microscopy
antisense oligonucleotides

Software Mentioned

GraphPad
BLAST
Prism
Primer3
Pick Primer tool

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