Peripheral infrastructure vectors and an extended set of plant parts for the Modular Cloning system

PloS One
Johannes GantnerJohannes Stuttmann

Abstract

Standardized DNA assembly strategies facilitate the generation of multigene constructs from collections of building blocks in plant synthetic biology. A common syntax for hierarchical DNA assembly following the Golden Gate principle employing Type IIs restriction endonucleases was recently developed, and underlies the Modular Cloning and GoldenBraid systems. In these systems, transcriptional units and/or multigene constructs are assembled from libraries of standardized building blocks, also referred to as phytobricks, in several hierarchical levels and by iterative Golden Gate reactions. Here, a toolkit containing further modules for the novel DNA assembly standards was developed. Intended for use with Modular Cloning, most modules are also compatible with GoldenBraid. Firstly, a collection of approximately 80 additional phytobricks is provided, comprising e.g. modules for inducible expression systems, promoters or epitope tags. Furthermore, DNA modules were developed for connecting Modular Cloning and Gateway cloning, either for toggling between systems or for standardized Gateway destination vector assembly. Finally, first instances of a "peripheral infrastructure" around Modular Cloning are presented: While available toolkit...Continue Reading

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Citations

Jan 27, 2019·Plant Cell Reports·Nicolas M DollThomas Widiez
Jul 14, 2019·Scientific Reports·David ChiassonMartin Parniske
Nov 9, 2018·Journal of Experimental Botany·Dipannita MitraKatharina Bürstenbinder
Dec 15, 2019·The Plant Journal : for Cell and Molecular Biology·Arianne SchnabelThomas Vogt
Dec 11, 2020·Metabolic Engineering·Rosanna YoungPaul S Freemont
Feb 3, 2021·Trends in Biotechnology·Roberto Di BlasiFrancesca Ceroni
Feb 26, 2021·The Plant Journal : for Cell and Molecular Biology·Jana OrdonJohannes Stuttmann
Feb 13, 2021·The Plant Journal : for Cell and Molecular Biology·Johannes StuttmannUlla Bonas
May 26, 2021·Nature Plants·Pratibha KumariKatharina Bürstenbinder
Aug 22, 2021·Current Biology : CB·M Ackerman-LavertS Savaldi-Goldstein

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Methods Mentioned

BETA
PCR
two hybrid
transgenic
three hybrid
Y2H
restriction digests
bacterial translocation
gene knockout
FRET

Software Mentioned

Plant Parts
Gateway
Modular Cloning
Addgene
Modular Cloning Toolkit
GreenGate
DEX
GoldenBraid
Modular

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