Peripheral leukocyte response to oncological radiotherapy: expression of heat shock proteins

International Journal of Radiation Biology
M C GuisasolaP García-Barreno

Abstract

To study Heat Shock Proteins (HSP) expression in patients subjected to radiotherapy and their potential use as biomarkers for radiation tolerance. An evaluation is also made of whether irradiated volume is critical to the outcome of normal tissue injury using polymorphonuclear neutrophils as biosensors, and whether HSP antibodies (Ab) may be involved in post-radiotherapy disease. Twelve patients receiving the same total dose of radiotherapy, but in three different volumes, and four healthy volunteers used as controls were analysed. hsp27 and 70i mRNA were determined by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and Southern-blot, HSP by flow cytometry, and HSP-Ab by Enzyme-linked Immnoadsorbent Assay (ELISA). The clinical protocol included radiation related toxicity based on clinical and analytical scales. Radiotherapy caused hsp downregulation, maximum in patients with the largest irradiated volumes, and a decrease in intracellular HSP content. Patients with greatest intraleukocyte HSP levels before treatment suffered more severe radiation morbidity. Patients with endocrine neoplasms presented the highest HSP-Ab titers. Radiotherapy downregulates hsp27 and 70i, which would enhance radiosensitivity. HSP content pr...Continue Reading

References

Sep 1, 1995·International Journal of Radiation Biology·H S MelkonyanS R Umansky
Apr 1, 1995·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology
Mar 30, 1995·International Journal of Radiation Oncology, Biology, Physics·J D CoxT F Pajak
Jan 1, 1994·Annual Review of Immunology·P Matzinger
Nov 1, 1995·Trends in Biochemical Sciences·P N Hengen
Jan 1, 1997·Journal of Clinical Pathology·S Frist
Mar 4, 1999·Gynecologic Oncology·J P GeislerZ Zhou
Sep 3, 1999·Nature Biotechnology·W P Halford
Sep 10, 1999·Annals of Medicine·M Jäättelä
Jun 1, 2000·Laboratory Investigation; a Journal of Technical Methods and Pathology·V Witko-SarsatL Halbwachs-Mecarelli
Aug 12, 2000·Free Radical Biology & Medicine·J PengK Watson
Aug 29, 2000·The Prostate·N B GibbonsJ M Fitzpatrick
Feb 15, 2001·International Journal of Radiation Oncology, Biology, Physics·B Sanchez-NietoD P Dearnaley
Mar 7, 2001·The Journal of Trauma·N HashiguchiH Sugimoto
Oct 2, 2001·Journal of Neurochemistry·O Goldbaum, C Richter-Landsberg
Oct 16, 2001·Cell Stress & Chaperones·P R Clark, A Ménoret
Dec 12, 2001·Journal of Clinical Densitometry : the Official Journal of the International Society for Clinical Densitometry·A SerraclaraF Hawkins
Jan 5, 2002·The American Journal of Medicine·W J Welch
Jan 16, 2002·Current Opinion in Immunology·Zihai LiPramod Srivastava
Feb 28, 2002·Circulation·A Graham Pockley
Apr 17, 2002·International Journal of Radiation Oncology, Biology, Physics·Jean-Denis LégalClaude Parmentier
Sep 7, 2002·International Journal of Cancer. Journal International Du Cancer·Heba Sh KassemGeoffrey P Margison
Oct 16, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Qingbo Xu
Dec 11, 2002·Immunological Investigations·Judit KocsisZoltán Prohászka
Jan 8, 2003·International Journal of Cancer. Journal International Du Cancer·Johanna HaikonenReidar Grénman
Jan 31, 2003·International Journal of Radiation Biology·J McIlrathE G Wright
Aug 2, 2003·Cancer Detection and Prevention·Li ZhongEdward A Hirschowitz
Oct 31, 2003·Expert Reviews in Molecular Medicine·A G Pockley
Nov 20, 2003·Methods : a Companion to Methods in Enzymology·Xiang-Yang WangJohn R Subjeck
Jan 1, 2004·Molecular and Cellular Biology·Clayton R HuntTej K Pandita
Mar 3, 2004·Surgical Oncology Clinics of North America·William F Sindelar, Timothy J Kinsella
Jan 1, 2005·Biophysical Journal·Theodore R RiegerVassily Hatzimanikatis

❮ Previous
Next ❯

Citations

Feb 15, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Xiaofeng LiJun Shu
May 23, 2012·International Journal of Radiation Oncology, Biology, Physics·Ting XuZhongxing Liao
Apr 10, 2007·Clinical Oncology : a Journal of the Royal College of Radiologists·C M L WestN G Burnet
Sep 23, 2011·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Jose Luis Lopez GuerraZhongxing Liao
Feb 5, 2013·International Journal of Radiation Oncology, Biology, Physics·Qingsong PangZhongxing Liao

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Polyendocrine Syndromes

This feed focuses on a rare genetic condition called Autoimmune Polyendocrine Syndromes, which are characterized by autoantibodies against multiple endocrine organs. This can lead to Type I Diabetes.

Biosensors for Cancer Detection

Biosensors are devices that are designed to detect a specific biological analyte by essentially converting a biological entity (ie, protein, DNA, RNA) into an electrical signal that can be detected and analyzed. The use of biosensors in cancer detection and monitoring holds vast potential. Biosensors can be designed to detect emerging cancer biomarkers and to determine drug effectiveness at various target sites. Biosensor technology has the potential to provide fast and accurate detection, reliable imaging of cancer cells, and monitoring of angiogenesis and cancer metastasis, and the ability to determine the effectiveness of anticancer chemotherapy agents.

Related Papers

Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology
Wong Kein LowDe Yun Wang
Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology
Richard P HillRobert S Bell
Journal of Human Nutrition and Dietetics : the Official Journal of the British Dietetic Association
J AbayomiG Bagnall
© 2022 Meta ULC. All rights reserved