Peripheral N-methyl-D-aspartate receptor localization and role in gastric acid secretion regulation: immunofluorescence and pharmacological studies

Scientific Reports
Iuliia GolovynskaTymish Y Ohulchanskyy

Abstract

The enteric nervous system (ENS) and a glutamate receptor (GluR), N-methyl-D-aspartate receptor (NMDAR), participate in gastric acid secretion (GAS) regulation. NMDARs are localized in different stomach cells; however, knowledge of NMDAR expression and function in the ENS is limited. In the present study, we clarified the types of stomach cells that express the NMDARs that are involved in GAS regulation. The pharmacological method of isolated stomach perfusion by Ghosh and Shild combined with direct mapping of NMDARs by fluorescence microscopy in the rat stomach was employed. By immunofluorescence labeling with an anti-NMDA-NR1 antibody, NMDARs were found to be highly expressed in nerve cells of the submucosal and myenteric plexuses in the stomach. The exact localization of the NMDARs relevant to GAS and its mechanism of action were determined by stimulating different receptors of neuronal and stomach cells using specific secretagogues for NMDA and by selectively blocking those receptors. NMDARs relevant to GAS stimulation are mainly localized in cholinergic interneurons; however, all of the nerve cells of the submucosal ganglia are involved in the stimulating process. In addition, the NMDARs in parietal cells are involved in g...Continue Reading

References

Dec 24, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E WeruagaJ Aijón
Apr 3, 2001·Trends in Pharmacological Sciences·T M Skerry, P G Genever
May 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·W RzeskiC Ikonomidou
Mar 1, 1958·British Journal of Pharmacology and Chemotherapy·M N GHOSH, H O SCHILD
Apr 6, 2004·Progress in Neurobiology·John B FurnessNadine Clerc
Sep 8, 2004·Journal of Neuroscience Research·Li Hsueh TsaiJang-Yen Wu
Feb 5, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Takeshi AiharaSusumu Okabe
May 27, 2005·Journal of Biomedical Science·Li Hsueh Tsai
Jul 27, 2005·The Journal of Experimental Biology·A L ObaidB M Salzberg
Aug 3, 2006·Molecular Pharmacology·Danyan MaoKenneth J Kellar
Jul 2, 2008·Journal of Gastroenterology·Kanako WatanabeTomoyuki Nishizaki
Jan 14, 2009·Phytotherapy Research : PTR·Pallab MaityUday Bandyopadhyay
Jan 23, 2010·Journal of Pharmacological Sciences·Eiji NakamuraKunio Torii
Oct 1, 2011·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Carrie K JonesMichael Bubser
Oct 16, 2013·Journal of Biophotonics·Ying-Ying HuangMichael R Hamblin
Apr 23, 2014·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Stephen I DeutschAndrew D Benson
Sep 17, 2015·Current Opinion in Gastroenterology·Mitchell L Schubert
Apr 7, 2016·Journal of Gastroenterology·Tadayuki Oshima, Hiroto Miwa
Jul 28, 2016·Journal of Gastroenterology·Mingming SunYingzi Cong
Aug 18, 2016·Helicobacter·Marino VeneritoPeter Malfertheiner
Sep 4, 2016·Current Opinion in Pharmacology·Yoku HayakawaTimothy C Wang
Jan 4, 2017·Scientific Reports·Tse-Ya YuHung-Yuan Li
Jan 27, 2017·Current Topics in Microbiology and Immunology·Adam J Smolka, Mitchell L Schubert

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fluorescence microscopy

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