Peripheral nerve lengthening by controlled isolated distraction: a new animal model

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
M W KroeberE Liebenberg

Abstract

We have developed a simple and effective animal model to study the distraction neurogenesis utilizing the sciatic nerve-lengthening technique in rats. The model allows macroscopic, physiological, and histological evaluation of the distraction site. Fourteen adult Harlan Sprague Dawley rats (300-350 g) were used in this study. A 10 mm segment of the right sciatic nerve of each animal in the nerve-lengthening group was resected. Gradual nerve lengthening was performed by advancing the proximal nerve stump at a rate of 1 mm/day. The proximal stump neuroma was then resected and a direct nerve anastomosis was performed. On the left side a standard autogenous nerve-grafting procedure was performed with a 10 mm segment of sciatic nerve used as an in situ nerve graft. Three months after the second surgery, the sciatic nerves were exposed and investigated by gross observation and EMG followed by histological processing and tissue analysis. Neomicrovascularization was observed surrounding the sciatic nerve anastomosis in all five specimens of the nerve-lengthening group as compared to the more white-colored scar tissue that was observed in the nerve-grafting group. The EMG results were similar for both groups. Histological studies of the...Continue Reading

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Citations

Nov 26, 2003·Experimental Neurology·Yuki HaraNaoyuki Ochiai
Jun 15, 2011·Journal of Neurotrauma·Joseph R LoverdeBryan J Pfister
Oct 15, 2003·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·Ichiro AbeNaoyuki Ochiai
Nov 6, 2008·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Ichiro AbeNaoyuki Ochiai
Jun 15, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Yuki HaraHajime Mishima
Jul 2, 2011·Journal of Nippon Medical School = Nippon Ika Daigaku Zasshi·Norie KoderaHiromoto Ito
Dec 6, 2003·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Yuki HaraNaoyuki Ochiai
Apr 5, 2011·Plastic and Reconstructive Surgery·Christine RadtkeKerstin Reimers
Oct 3, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·UNKNOWN SaijilafuNaoyuki Ochiai
Mar 11, 2008·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·UNKNOWN SaijilafuNaoyuki Ochiai
Jul 7, 2007·The Journal of Bone and Joint Surgery. British Volume·Y HaraY Nishiura
Dec 10, 2009·Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery·Yasutaka YamadaNaoyuki Ochiai

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